ZFIN ID: ZDB-FIG-130326-48
Djiotsa et al., 2012 - Pax4 is not essential for beta-cell differentiation in zebrafish embryos but modulates alpha-cell generation by repressing arx gene expression. BMC Developmental Biology   12(1):37 Full text @ BMC Dev. Biol.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Knockdown Reagent:
Anatomical Terms:
Stage: Prim-15
PHENOTYPE:
Fish:
Knockdown Reagent:
Observed In:
Stage: Prim-15

Fig. 5 Pax4 is not required for β-cell differentiation. (A - J) Ventral views of the pancreas area from embryos analyzed by WISH, anterior to the left. Pancreatic expression of insulin (A, B), somatostatin (C, D), ghrelin (E, F), glucagon (G, H) and arx (I, J) in control morphants (A, C, E, F, I) and pax4 morphants (B, D, F, G, J) at 30 hpf. Quantifications (K, L, M, N) represent the number of positive cells per embryo for control morphants and pax4 morphants. Asterisks (*) indicate that the difference between cell number in control morphants and pax4 morphants is statistically significant by Student’s t-test (**: P<0.003; ***: P<0.0001). Original magnification 400x.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
arxa WT control Prim-15 pancreatic bud ISH
Prim-15 posterior pancreatic bud ISH
WT + MO1-pax4 standard conditions Prim-15 pancreatic bud ISH
Prim-15 posterior pancreatic bud ISH
gcga WT control Prim-15 glucagon secreting cell ISH
Prim-15 pancreatic A cell ISH
WT + MO1-pax4 standard conditions Prim-15 glucagon secreting cell ISH
Prim-15 pancreatic A cell ISH
ghrl WT control Prim-15 ghrelin secreting cell ISH
Prim-15 pancreatic epsilon cell ISH
WT + MO1-pax4 standard conditions Prim-15 ghrelin secreting cell ISH
Prim-15 pancreatic epsilon cell ISH
ins WT control Prim-15 insulin secreting cell ISH
Prim-15 pancreatic B cell ISH
WT + MO1-pax4 standard conditions Prim-15 insulin secreting cell ISH
Prim-15 pancreatic B cell ISH
sst2 WT control Prim-15 D cell ISH
Prim-15 somatostatin secreting cell ISH
WT + MO1-pax4 standard conditions Prim-15 D cell ISH
Prim-15 somatostatin secreting cell ISH
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
WT + MO1-pax4 standard conditions Prim-15 ghrelin secreting cell increased amount, abnormal
Prim-15 glucagon secreting cell increased amount, abnormal
Prim-15 pancreatic A cell increased amount, abnormal
Prim-15 pancreatic epsilon cell increased amount, abnormal
Acknowledgments:
ZFIN wishes to thank the journal BMC Developmental Biology for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ BMC Dev. Biol.