ZFIN ID: ZDB-FIG-130326-51
Djiotsa et al., 2012 - Pax4 is not essential for beta-cell differentiation in zebrafish embryos but modulates alpha-cell generation by repressing arx gene expression. BMC Developmental Biology   12(1):37 Full text @ BMC Dev. Biol.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Knockdown Reagents:
Anatomical Terms:
Stage: Prim-15

Fig. 8 Identity of pax4+ and arx+ cells following Pax4 and Arx inactivation. (A F) Double fluorescent WISH performed on 30 hpf embryos showing expression of pax4 transcripts (green) in the different pancreatic cell types of control morphants (A, C, E, G) and in pax4 morphants (B, D, F, H). All panels display ventral vues except panels D and H which are lateral vues. (IN) Confocal images of pancreatic area with arx probe and various pancreatic hormones at 30 hpf in control (I-L) and double pax4/arx morphants (M-P). Anterior part to the left. Scale bar = 20 μm. Asterisks (*) indicate double positive cells.

Gene Expression Details
Gene Antibody Fish Conditions Stage Qualifier Anatomy Assay
arxa WT control Prim-15 Not Detected D cell ISH
Prim-15 ghrelin secreting cell ISH
Prim-15 Not Detected insulin secreting cell ISH
Prim-15 Not Detected pancreatic B cell ISH
Prim-15 pancreatic epsilon cell ISH
Prim-15 Not Detected somatostatin secreting cell ISH
WT + MO1-pax4 + MO3-arxa standard conditions Prim-15 Not Detected D cell ISH
Prim-15 ghrelin secreting cell ISH
Prim-15 Not Detected insulin secreting cell ISH
Prim-15 Not Detected pancreatic B cell ISH
Prim-15 pancreatic epsilon cell ISH
Prim-15 Not Detected somatostatin secreting cell ISH
gcga WT control Prim-15 glucagon secreting cell ISH
Prim-15 pancreatic A cell ISH
WT + MO1-pax4 standard conditions Prim-15 glucagon secreting cell ISH
Prim-15 pancreatic A cell ISH
WT + MO1-pax4 + MO3-arxa standard conditions Prim-15 Not Detected glucagon secreting cell ISH
Prim-15 Not Detected pancreatic A cell ISH
ghrl WT control Prim-15 ghrelin secreting cell ISH
Prim-15 pancreatic epsilon cell ISH
WT + MO1-pax4 + MO3-arxa standard conditions Prim-15 Not Detected D cell ISH
Prim-15 ghrelin secreting cell ISH
Prim-15 Not Detected insulin secreting cell ISH
Prim-15 Not Detected pancreatic B cell ISH
Prim-15 pancreatic epsilon cell ISH
Prim-15 Not Detected somatostatin secreting cell ISH
ins WT control Prim-15 insulin secreting cell ISH
Prim-15 pancreatic B cell ISH
WT + MO1-pax4 standard conditions Prim-15 insulin secreting cell ISH
Prim-15 pancreatic B cell ISH
WT + MO1-pax4 + MO3-arxa standard conditions Prim-15 insulin secreting cell ISH
Prim-15 pancreatic B cell ISH
pax4 WT control Prim-15 D cell ISH
Prim-15 ghrelin secreting cell ISH
Prim-15 Not Detected glucagon secreting cell ISH
Prim-15 Not Detected pancreatic A cell ISH
Prim-15 pancreatic epsilon cell ISH
Prim-15 posterior pancreatic bud ISH
Prim-15 somatostatin secreting cell ISH
WT + MO1-pax4 standard conditions Prim-15 D cell ISH
Prim-15 ghrelin secreting cell ISH
Prim-15 glucagon secreting cell ISH
Prim-15 pancreatic A cell ISH
Prim-15 pancreatic epsilon cell ISH
Prim-15 posterior pancreatic bud ISH
Prim-15 somatostatin secreting cell ISH
sox4b WT standard conditions Prim-15 posterior pancreatic bud ISH
WT + MO1-pax4 standard conditions Prim-15 posterior pancreatic bud ISH
sst2 WT control Prim-15 D cell ISH
Prim-15 somatostatin secreting cell ISH
WT + MO1-pax4 standard conditions Prim-15 D cell ISH
Prim-15 somatostatin secreting cell ISH
WT + MO1-pax4 + MO3-arxa standard conditions Prim-15 D cell ISH
Prim-15 somatostatin secreting cell ISH
Antibody Labeling Details No data available
Phenotype Details No data available
Acknowledgments:
ZFIN wishes to thank the journal BMC Developmental Biology for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ BMC Dev. Biol.