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ZFIN ID: ZDB-FIG-130222-2
Li et al., 2011 - Histone H4 Lys 20 monomethylation by histone methylase SET8 mediates Wnt target gene activation. Proceedings of the National Academy of Sciences of the United States of America   108(8):3116-3123 Full text @ Proc. Natl. Acad. Sci. USA
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Knockdown Reagents:
Anatomical Terms:
Stage: Shield
PHENOTYPE:
Fish:
Knockdown Reagents:
Observed In:
Stage: Shield

Fig. 4

SET8 regulates Wnt target genes in zebrafish. (A and B) Knockdown of set8a resulted in dorsal mesodermal expansion. Embryos injected with the indicated MOs were fixed at the shield stage and stained for tbx6, cdx4, gsc, and ntl. The graph shows the statistical data for each gene, respectively. (C) set8a MO showed synergistic effect with wnt8 MOs on Wnt responsive genes. The graph shows the statistical data of the in situ experiment for each gene, respectively. (D) Set8a regulated cdx4 and tbx6 expression. The embryos injected MOs, as indicated, were collected at the shield stage. The cdx4 and tbx6 expression was detected by quantitative real-time PCR and normalized by ntl gene expression. (E) set8a MO and wnt8 MOs reduced the transcription level of cdx4 and tbx6, both alone and synergistically. **P < 0.01; *P < 0.05.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
cdx4 TU control Shield margin ISH
Shield whole organism RTPCR
TU + MO1-kmt5aa standard conditions Shield margin ISH
Shield whole organism RTPCR
TU + MO1-kmt5aa + MO1-wnt8a + MO2-wnt8a standard conditions Shield whole organism RTPCR
TU + MO1-wnt8a + MO2-wnt8a standard conditions Shield margin ventral region ISH
Shield whole organism RTPCR
gsc TU control Shield shield ISH
TU + MO1-kmt5aa standard conditions Shield shield ISH
TU + MO1-wnt8a + MO2-wnt8a standard conditions Shield shield ISH
tbx16l TU control Shield margin ventral region ISH
Shield whole organism RTPCR
TU + MO1-kmt5aa standard conditions Shield margin ventral region ISH
Shield whole organism RTPCR
TU + MO1-kmt5aa + MO1-wnt8a + MO2-wnt8a standard conditions Shield whole organism RTPCR
TU + MO1-wnt8a + MO2-wnt8a standard conditions Shield margin ventral region ISH
Shield whole organism RTPCR
tbxta TU control Shield margin ISH
TU + MO1-kmt5aa standard conditions Shield margin ISH
TU + MO1-wnt8a + MO2-wnt8a standard conditions Shield margin ISH
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
TU + MO1-kmt5aa standard conditions Shield shield increased width, abnormal
TU + MO1-wnt8a + MO2-wnt8a standard conditions Shield shield increased width, abnormal
Acknowledgments:
ZFIN wishes to thank the journal Proceedings of the National Academy of Sciences of the United States of America for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Proc. Natl. Acad. Sci. USA