ZFIN ID: ZDB-FIG-130222-11
Li et al., 2011 - Histone H4 Lys 20 monomethylation by histone methylase SET8 mediates Wnt target gene activation. Proceedings of the National Academy of Sciences of the United States of America   108(8):3116-3123 Full text @ Proc. Natl. Acad. Sci. USA
ADDITIONAL FIGURES
PHENOTYPE:
Fish:
Knockdown Reagent:
Observed In:
Stage: Shield

Fig. S4

The ATG-MO set8a MO2 displays similar results as the set8a UTR-MO. (A) Injection of SET8-114 mRNA rescued the set8a morphant phenotype. The morphants were classified into four categories: 0, S1, S2, and S3. (B) The set8a MO2 acted synergistically with the wnt8 MOs. The morphants were classified into four categories: 0, W1, W2, and W3. (C) Injection of SET8-114 mRNA but not the 114H299A mutant rescued the synergistic effect of set8a MO2 and wnt8 MOs. (D) set8a MO2 injection led to dorsal mesoderm expansion, which could be rescued by SET8-114 mRNA. Embryos injected with the indicated morpholinos were fixed at the shield stage and stained for tbx6, cdx4, gsc, and ntl. The graph shows the statistical data for each gene, respectively.

Gene Expression Details No data available
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
TU + MO2-kmt5aa standard conditions Shield shield increased width, abnormal
Acknowledgments:
ZFIN wishes to thank the journal Proceedings of the National Academy of Sciences of the United States of America for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Proc. Natl. Acad. Sci. USA