The ATG-MO set8a MO2 displays similar results as the set8a UTR-MO. (A) Injection of SET8-114 mRNA rescued the set8a morphant phenotype. The morphants were classified into four categories: 0, S1, S2, and S3. (B) The set8a MO2 acted synergistically with the wnt8 MOs. The morphants were classified into four categories: 0, W1, W2, and W3. (C) Injection of SET8-114 mRNA but not the 114H299A mutant rescued the synergistic effect of set8a MO2 and wnt8 MOs. (D) set8a MO2 injection led to dorsal mesoderm expansion, which could be rescued by SET8-114 mRNA. Embryos injected with the indicated morpholinos were fixed at the shield stage and stained for tbx6, cdx4, gsc, and ntl. The graph shows the statistical data for each gene, respectively.