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ZFIN ID: ZDB-FIG-130222-1
Li et al., 2011 - Histone H4 Lys 20 monomethylation by histone methylase SET8 mediates Wnt target gene activation. Proceedings of the National Academy of Sciences of the United States of America   108(8):3116-3123 Full text @ Proc. Natl. Acad. Sci. USA
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Gene:
Fish:
Knockdown Reagents:
Anatomical Term:
Stage: 10-13 somites
PHENOTYPE:
Fish:
Knockdown Reagents:
Observed In:
Stage Range: Shield to Prim-5

Fig. 3

Set8a participates in Wnt signaling to regulate zebrafish embryonic development. (A) set8a MO led to H4K20me-1 reduction at tbx6 TBE. Zebrafish embryos were injected with distinct set8a or wnt8 MOs at one-cell stage and collected at shield stage for ChIP experiment. Quantitative PCR results of IgG were set as 1. P values were calculated between control MO and set8a or wnt8 MO injection. **P < 0.01. (B) Morphology of set8a (0, S1, S2, and S3) and wnt8 (0, W1, W2, and W3) morphants at 26 hpf. The wnt8 morphants were classified into four categories, with 0 being WT and W3 being the most severe phenotype. The set8a morphants were classified into four categories, with S1 displaying relatively small head, S2 displaying small head and shortened trunk and tail, and S3 displaying small head and severely shortened trunk and tail. (C) SET8-114 mRNA restored the set8a MO phenotype. The number of embryos scored (N) is shown on top of each bar, and the concentration of MO is indicated. The morphant phenotypes were classified into four categories: 0, S1, S2, and S3. (D) set8a MO enhanced the neuroecodermal and mesodermal defect of wnt8 MOs. Embryos were examined at the 10-somite stage. In situ markers used were opl (telencephalon), pax2.1 (midbrain/hindbrain boundary), and myod (adaxial cells and somites). Red arrowheads, opl expression; black arrowheads, notocord. (E) SET8-114 mRNA but not 114H299A rescued the synergistic effect of set8a MO and wnt8 MOs. The morphants phenotypes were classified into four categories: 0, W1, W2, and W3.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
zic1 TU standard conditions 10-13 somites telencephalon ISH
TU + MO1-kmt5aa standard conditions 10-13 somites telencephalon ISH
TU + MO1-kmt5aa + MO1-wnt8a + MO2-wnt8a standard conditions 10-13 somites telencephalon ISH
TU + MO1-wnt8a + MO2-wnt8a standard conditions 10-13 somites telencephalon ISH
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
TU + MO1-kmt5aa standard conditions Shield histone methyltransferase activity (H4-K20 specific) disrupted, abnormal
10-13 somites post-vent region decreased length, abnormal
10-13 somites telencephalon increased size, abnormal
10-13 somites trunk decreased length, abnormal
Prim-5 post-vent region decreased length, abnormal
Prim-5 trunk decreased length, abnormal
TU + MO1-kmt5aa + MO1-wnt8a + MO2-wnt8a standard conditions 10-13 somites post-vent region decreased length, abnormal
10-13 somites telencephalon increased size, abnormal
10-13 somites trunk decreased length, abnormal
TU + MO1-wnt8a + MO2-wnt8a standard conditions Shield histone methyltransferase activity (H4-K20 specific) disrupted, abnormal
10-13 somites post-vent region decreased length, abnormal
10-13 somites telencephalon increased size, abnormal
10-13 somites trunk decreased length, abnormal
Prim-5 post-vent region decreased length, abnormal
Prim-5 trunk decreased length, abnormal
TU + MO2-kmt5aa standard conditions Shield histone methyltransferase activity (H4-K20 specific) disrupted, abnormal
Acknowledgments:
ZFIN wishes to thank the journal Proceedings of the National Academy of Sciences of the United States of America for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Proc. Natl. Acad. Sci. USA