FIGURE

Fig. 1

ID

ZDB-FIG-120601-100

Publication

Cai et al., 2012 - Cellular retinoic acid-binding proteins are essential for hindbrain patterning and signal robustness in zebrafish

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Fig. 1

crabp2a and crabp2b are required for zebrafish hindbrain patterning. (A-L) Whole-mount in situ hybridization (ISH) for pax2a at the midbrain-hindbrain boundary (MHB), krox20 in rhombomere (R) 3 and R5, and hoxb4 (A-H) or hoxb5 (I-L) in R7 and spinal cord at 18 hpf; dorsal views, anterior left. Compared with uninjected controls (A), morpholino (MO) depletion of Crabp2a (B,J) or Crabp2b (C,K) slightly reduces and co-injection (D,L) eliminates hoxb4 and hoxb5 expression (arrows). Exogenous RA treatments (5-20 nM) that posteriorize the hindbrain do not rescue hoxb4 expression (E-H). wt, wild type.

Expression Data

Genes:	egr2b hoxb4a hoxb5a pax2a
Fish:	WT WT + MO1-crabp2a WT + MO1-crabp2b WT + MO1-crabp2a + MO1-crabp2b
Condition:	chemical treatment: all-trans-retinoic acid
Knockdown Reagents:	MO1-crabp2a MO1-crabp2b
Anatomical Terms:	midbrain hindbrain boundary rhombomere 3 rhombomere 5 rhombomere 7 spinal cord neural tube
Stage:	14-19 somites

Expression Detail

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Phenotype Data

Fish:	WT WT + MO1-crabp2a + MO1-crabp2b
Condition:	chemical treatment: all-trans-retinoic acid
Knockdown Reagents:	MO1-crabp2a MO1-crabp2b
Observed In:	hindbrain hindbrain morphogenesis retinoic acid receptor signaling pathway rhombomere 7 spinal cord neural tube
Stage:	14-19 somites

Phenotype Detail

Acknowledgments

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