FIGURE

Fig. S6

ID

ZDB-FIG-120601-98

Publication

Cai et al., 2012 - Cellular retinoic acid-binding proteins are essential for hindbrain patterning and signal robustness in zebrafish

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Fig. S6

Increased RARE:YFP expression in response to exogenous RA after crabp2a and crabp2b knockdown. Live Tg(RARE-gata2:NTD-eYFP)Id1 transgenics, dorsal views of the hindbrain, anterior to the left. (A,B) Expression extends just anterior to the level of somite 1 (s1) in DMSO-treated controls (A) and extends further anteriorly <100 μm after treatment during gastrulation with 1 nM exogenous RA (B). (C,D) crabp2a/crabp2b knockdown alone slightly reduces Tg(RARE-gata2:NTD-eYFP)Id1 expression (C), but when combined with 1 nm RA expression expands throughout the CNS (D). ′o′ indicates approximate location of the otic vesicle.

Expression Data

Gene:	EYFP
Fish:	ld1Tg ld1Tg + MO1-crabp2a + MO1-crabp2b
Condition:	chemical treatment: all-trans-retinoic acid
Knockdown Reagents:	MO1-crabp2a MO1-crabp2b
Anatomical Terms:	central nervous system hindbrain hindbrain neural tube posterior region spinal cord neural tube anterior region
Stage:	20-25 somites

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	ld1Tg + MO1-crabp2a + MO1-crabp2b
Condition:	chemical treatment: all-trans-retinoic acid
Knockdown Reagents:	MO1-crabp2a MO1-crabp2b
Observed In:	hindbrain morphogenesis retinoic acid receptor signaling pathway
Stage:	20-25 somites

Phenotype Detail

Acknowledgments

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