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Fig. 1 crabp2a and crabp2b are required for zebrafish hindbrain patterning. (A-L) Whole-mount in situ hybridization (ISH) for pax2a at the midbrain-hindbrain boundary (MHB), krox20 in rhombomere (R) 3 and R5, and hoxb4 (A-H) or hoxb5 (I-L) in R7 and spinal cord at 18 hpf; dorsal views, anterior left. Compared with uninjected controls (A), morpholino (MO) depletion of Crabp2a (B,J) or Crabp2b (C,K) slightly reduces and co-injection (D,L) eliminates hoxb4 and hoxb5 expression (arrows). Exogenous RA treatments (5-20 nM) that posteriorize the hindbrain do not rescue hoxb4 expression (E-H). wt, wild type.