ZFIN ID: ZDB-FIG-120209-7
Hall et al., 2012 - Infection-Responsive Expansion of the Hematopoietic Stem and Progenitor Cell Compartment in Zebrafish Is Dependent upon Inducible Nitric Oxide. Cell Stem Cell   10(2):198-209 Full text @ Cell Stem Cell
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Condition:
Anatomical Terms:
Stage: Day 4
PHENOTYPE:
Fish:
Condition:
Observed In:
Stage: Day 4

Fig. 1

Larval Hematopoietic Response to Infection (A) Schematic illustrating hindbrain injection site of live GFP-labeled Salmonella. (B) Quantities of whole-larvae lyz:DsRED-expressing cells (per 100,000 sorted cells), as determined by flow cytometry, within infected Tg(lyz:DsRED) larvae (600 cfu) at 1, 2, 3, and 5 dpi, compared with PBS controls (n = 50 larvae/sample). (C and D) Enhanced granulopoiesis within the AGM and CHT of infected (600 cfu) Tg(lyz:EGFP)/Tg(gata1:DsRED) larvae at 2 dpi, relative to PBS controls (with magnified views of AGM region within similarly treated Tg(lyz:DsRED) larvae). (B)–(D) refer only to those infected larvae that displayed neutrophil depletion at 1 dpi (see Supplemental Experimental Procedures). (E) Expression of mpeg1 and spi1 within the AGM at 2 dpi following infection, compared with PBS controls. Inset demonstrates mpeg1-expressing cells within the midbrain and hindbrain. (F) Dual WMISH of lyz and spi1 expression within the AGM following infection at 2 dpi. All views, anterior to left. Scale bar, 100 μm in (D). Abbreviations: AGM, aorta-gonad-mesonephros; CHT, caudal hematopoietic tissue; DA, dorsal aorta; PCV, posterior cardinal vein. See also Figure S1.

Gene Expression Details
Gene Antibody Fish Conditions Stage Qualifier Anatomy Assay
DsRed nz50Tg standard conditions Day 4 ventral wall of dorsal aorta neutrophil IFL
nz50Tg bacterial treatment: Salmonella enterica subsp. enterica serovar Typhimurium Day 4 ventral wall of dorsal aorta neutrophil IFL
EGFP nz117Tg; sd2Tg standard conditions Day 4 neutrophil IFL
Day 4 ventral wall of dorsal aorta neutrophil IFL
nz117Tg; sd2Tg bacterial treatment: Salmonella enterica subsp. enterica serovar Typhimurium Day 4 neutrophil IFL
Day 4 ventral wall of dorsal aorta neutrophil IFL
lyz WT bacterial treatment: Salmonella enterica subsp. enterica serovar Typhimurium Day 4 ventral wall of dorsal aorta neutrophil ISH
mpeg1.1 WT standard conditions Day 4 Not Detected ventral wall of dorsal aorta anatomical region ISH
WT bacterial treatment: Salmonella enterica subsp. enterica serovar Typhimurium Day 4 Not Detected ventral wall of dorsal aorta anatomical region ISH
spi1b WT standard conditions Day 4 Not Detected ventral wall of dorsal aorta anatomical region ISH
Day 4 Not Detected ventral wall of dorsal aorta granulocyte monocyte progenitor cell ISH
WT bacterial treatment: Salmonella enterica subsp. enterica serovar Typhimurium Day 4 ventral wall of dorsal aorta granulocyte monocyte progenitor cell ISH
Day 4 ventral wall of dorsal aorta neutrophil ISH
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
nz117Tg; sd2Tg bacterial treatment: Salmonella enterica subsp. enterica serovar Typhimurium Day 4 ventral wall of dorsal aorta neutrophil increased amount, abnormal
Acknowledgments:
ZFIN wishes to thank the journal Cell Stem Cell for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Cell Stem Cell