QPCR expression analysis of csf1a (A), csf1b (B) and gscf (C) at 1.25 and 2.25 dpi following infection, relative to PBS controls, means standard errors. (D) RT-PCR detection of disrupted gcsfr splicing at 2, 3 and 4 dpf following Gcsfr MO injection. (E) Effect of Gcsfr-depletion on steady-state granulopoiesis. Relative quantities of whole-larvae fluorescent cells, as determined by flow cytometry, within Tg(lyz:DsRED) larvae at 2, 3 and 4 dpf following injection of control and Gcsfr-targeting MOs, relative to uninjected siblings. (F and G) Gcsfr-depleted larvae demonstrate reduced survival to live, but not heat-killed, Salmonella. (F) Survival graph of control MO injected/PBS (n=10), control MO injected/250 cfu infected (n=26), control MO injected/500 cfu infected (n=25), Gcsfr MO injected/PBS (n=20), Gcsfr MO injected/250 cfu infected (n=34) and Gcsfr MO injected/500 cfu infected (n=35). (G) Survival graph of control MO injected/PBS (n=45), control MO injected/heat-shocked Salmonella (H.S. Sal.) injected (n=45), Gscfr MO injected/PBS (n=25) and Gcsfr MO injected/ H.S. Sal. injected (n=23). Abbreviations: n.s., not significant; *, p-value<0.05; ***, p-value<0.001.