ZFIN ID: ZDB-FIG-131217-2
Hall et al., 2012 - Infection-Responsive Expansion of the Hematopoietic Stem and Progenitor Cell Compartment in Zebrafish Is Dependent upon Inducible Nitric Oxide. Cell Stem Cell   10(2):198-209 Full text @ Cell Stem Cell
Knockdown Reagent:
Observed In:
Stage Range: 1-cell to Day 4

Fig. S3

QPCR expression analysis of csf1a (A), csf1b (B) and gscf (C) at 1.25 and 2.25 dpi following infection, relative to PBS controls, means standard errors. (D) RT-PCR detection of disrupted gcsfr splicing at 2, 3 and 4 dpf following Gcsfr MO injection. (E) Effect of Gcsfr-depletion on steady-state granulopoiesis. Relative quantities of whole-larvae fluorescent cells, as determined by flow cytometry, within Tg(lyz:DsRED) larvae at 2, 3 and 4 dpf following injection of control and Gcsfr-targeting MOs, relative to uninjected siblings. (F and G) Gcsfr-depleted larvae demonstrate reduced survival to live, but not heat-killed, Salmonella. (F) Survival graph of control MO injected/PBS (n=10), control MO injected/250 cfu infected (n=26), control MO injected/500 cfu infected (n=25), Gcsfr MO injected/PBS (n=20), Gcsfr MO injected/250 cfu infected (n=34) and Gcsfr MO injected/500 cfu infected (n=35). (G) Survival graph of control MO injected/PBS (n=45), control MO injected/heat-shocked Salmonella (H.S. Sal.) injected (n=45), Gscfr MO injected/PBS (n=25) and Gcsfr MO injected/ H.S. Sal. injected (n=23). Abbreviations: n.s., not significant; *, p-value<0.05; ***, p-value<0.001.

Gene Expression Details No data available
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
WT + MO4-csf3r bacterial treatment: Salmonella enterica subsp. enterica serovar Typhimurium 1-cell - Long-pec whole organism decreased life span, abnormal
nz50Tg + MO4-csf3r standard conditions Long-pec - Day 4 granulocyte differentiation decreased occurrence, abnormal
Long-pec - Day 4 neutrophil increased amount, abnormal
ZFIN wishes to thank the journal Cell Stem Cell for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Cell Stem Cell