ZFIN ID: ZDB-FIG-120209-11
Hall et al., 2012 - Infection-Responsive Expansion of the Hematopoietic Stem and Progenitor Cell Compartment in Zebrafish Is Dependent upon Inducible Nitric Oxide. Cell Stem Cell   10(2):198-209 Full text @ Cell Stem Cell
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Condition:
Knockdown Reagent:
Anatomical Terms:
Stage Range: Protruding-mouth to Day 4
PHENOTYPE:
Fish:
Condition:
Observed In:
Stage: Day 4

Fig. 5

Demand-Driven Granulopoiesis and HSPC Expansion Are Dependent upon Nos2a Relative frequency of infected larvae demonstrating demand-driven granulopoiesis following treatment (from 6 hpi) with the NOS inhibiting drugs L-NAME (A), ODQ (B), 1400W (C), and S-Methyl-L-thiocitrulline (D), all relative to DMSO control treatment. Numbers represent numbers of larvae in each treatment group. (E) Expression of nos2a within the AGM of infected larvae, compared with PBS controls at 1 and 2 dpi. (F) Dual WMISH of nos2a and lyz within the AGM of infected larvae at 2 dpi. (G–I) DAF-FM DA detection of NO within control MO-injected PBS control, control MO-injected infected, and Nos2a MO-injected infected Tg(lyz:DsRED) larvae, respectively, at 2 dpi. Yellow and red asterisks mark lyz+/NO+ and lyz+/NO* cells, respectively. (J) Percentage of control MO- and Nos2a MO-injected Tg(lyz:DsRED) infected larvae demonstrating demand-driven granulopoiesis at 2 dpi. (K) Number of cmyb-expressing HSPCs, as detected by WMISH at 2 dpi, within the AGM compartment (as defined in Figure 2C) following indicated treatments. (L) Quantification of dual-labeled HSPCs within the subaortic space of 2 dpi Tg(runx1P2:EGFP)/Tg(kdrl:nls-mCherry) larvae following indicated treatments. Numbers represent frequency of embryos or larvae with displayed phenotype. All views, anterior to left. Scale bar, 50 μm in (F). Abbreviations: DA, dorsal aorta; PCV, posterior cardinal vein; n.s., not significant; **p < 0.01; ***p < 0.001. See also Figure S5.

Gene Expression Details
Gene Antibody Fish Conditions Stage Qualifier Anatomy Assay
DsRed nz50Tg standard conditions Day 4 ventral wall of dorsal aorta neutrophil IFL
nz50Tg bacterial treatment: Salmonella enterica subsp. enterica serovar Typhimurium Day 4 ventral wall of dorsal aorta neutrophil IFL
nz50Tg + MO3-nos2a bacterial treatment: Salmonella enterica subsp. enterica serovar Typhimurium Day 4 ventral wall of dorsal aorta neutrophil IFL
lyz WT bacterial treatment: Salmonella enterica subsp. enterica serovar Typhimurium Day 4 ventral wall of dorsal aorta neutrophil ISH
nos2a WT standard conditions Protruding-mouth Not Detected ventral wall of dorsal aorta ISH
Day 4 Not Detected ventral wall of dorsal aorta ISH
WT bacterial treatment: Salmonella enterica subsp. enterica serovar Typhimurium Protruding-mouth ventral wall of dorsal aorta ISH
Day 4 ventral wall of dorsal aorta ISH
Day 4 ventral wall of dorsal aorta neutrophil ISH
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
nz50Tg bacterial treatment: Salmonella enterica subsp. enterica serovar Typhimurium Day 4 ventral wall of dorsal aorta neutrophil increased amount, abnormal
Acknowledgments:
ZFIN wishes to thank the journal Cell Stem Cell for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Cell Stem Cell