ZFIN ID: ZDB-FIG-120209-14
Hall et al., 2012 - Infection-Responsive Expansion of the Hematopoietic Stem and Progenitor Cell Compartment in Zebrafish Is Dependent upon Inducible Nitric Oxide. Cell Stem Cell   10(2):198-209 Full text @ Cell Stem Cell
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Condition:
Knockdown Reagent:
Anatomical Terms:
Stage Range: Protruding-mouth to Day 4
PHENOTYPE:
Fish:
Condition:
Knockdown Reagent:
Observed In:
Stage Range: Long-pec to Day 6

Fig. S2

(A) RT-PCR detection of disrupted runx1 splicing at 2 and 3 dpf following Runx1 MO injection. (B) Confocal images through the AGM of control MO- and Runx1 MO-injected Tg(runx1P2:EGFP) larvae at 3 dpf. (C) Survival graph of control MO injected/PBS (n=12), control MO injected/300 cfu infected (n=24), Runx1 MO injected/PBS (n=12) and Runx1 MO injected/300 cfu infected (n=24). (D) QPCR analysis of runx1 expression at 1.25 and 2.25 dpi, relative to PBS controls, means standard ± errors. (E) Effect of Runx1-depletion on steady-state granulopoiesis. Relative quantities of whole-larvae fluorescent cells, as determined by flow cytometry, within Tg(lyz:DsRED) larvae at 2, 3 and 4 dpf following injection of control and Runx1-targeting MOs, relative to uninjected siblings. (F) Confocal imaging through the AGM region of PBS control and Infected Tg(runx1P2:EGFP)/Tg(kdrl:nls-mCherry) larvae (1 dpi) revealing no significant change in the number of VDA-localized dual fluorescent (runx1+kdrl+) putative hemogenic endothelial cells (G), marked with asterisks. Arrowheads mark dual-labeled subaortic HSPCs. All views, anterior to left. Scale bar, 50m in B. Abbreviations: n.s., not significant; DA, dorsal aorta; VDA, ventral dorsal aorta; **, p-value<0.01.

Gene Expression Details
Gene Antibody Fish Conditions Stage Qualifier Anatomy Assay
EGFP nz10Tg; zf188Tg standard conditions Day 4 mesenchyme hematopoietic stem cell IFL
nz10Tg; zf188Tg bacterial treatment: Salmonella enterica subsp. enterica serovar Typhimurium Day 4 mesenchyme hematopoietic stem cell IFL
zf188Tg standard conditions Protruding-mouth ventral wall of dorsal aorta hematopoietic multipotent progenitor cell IFL
Protruding-mouth ventral wall of dorsal aorta hematopoietic stem cell IFL
zf188Tg + MO9-runx1 standard conditions Protruding-mouth Not Detected ventral wall of dorsal aorta hematopoietic multipotent progenitor cell IFL
Protruding-mouth Not Detected ventral wall of dorsal aorta hematopoietic stem cell IFL
mCherry nz10Tg; zf188Tg standard conditions Day 4 mesenchyme hematopoietic stem cell IFL
nz10Tg; zf188Tg bacterial treatment: Salmonella enterica subsp. enterica serovar Typhimurium Day 4 mesenchyme hematopoietic stem cell IFL
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
WT + MO9-runx1 bacterial treatment: Salmonella enterica subsp. enterica serovar Typhimurium Day 4 - Day 6 whole organism decreased life span, abnormal
WT + MO9-runx1 standard conditions Long-pec - Day 4 granulocyte differentiation decreased rate, abnormal
zf188Tg + MO9-runx1 standard conditions Protruding-mouth hematopoietic stem cell aplastic, abnormal
Acknowledgments:
ZFIN wishes to thank the journal Cell Stem Cell for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Cell Stem Cell