FIGURE

Fig. 2

ID

ZDB-FIG-120209-8

Publication

Hall et al., 2012 - Infection-Responsive Expansion of the Hematopoietic Stem and Progenitor Cell Compartment in Zebrafish Is Dependent upon Inducible Nitric Oxide

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Fig. 2

HSPC Compartment Expands in Response to Infection (A) Confocal imaging through the AGM of control MO- and Runx1 MO-injected Tg(lyz:DsRED)/Tg(runx1P2:EGFP) larvae following infection, compared with PBS controls at 2 dpi. (B) Expression of cmyb within the AGM of uninjected, PBS control and infected larvae at 2 dpi. (C) Quantification of cmyb-expressing cells within the AGM (as defined in schematic) of larvae shown in (B); arrowhead marks somite boundary that intersects cloaca. (D) Quantification of dual-labeled HSPCs (marked by asterisks) within the subaortic space of infected Tg(runx1P2:EGFP)/Tg(kdrl:nls-mCherry) larvae, compared with PBS controls at 1 and 2 dpi. All views, anterior to left. Numbers represent frequency of embryos or larvae with displayed phenotype. Scale bar, 50 μm in (A). Abbreviations: DA, dorsal aorta; PCV, posterior cardinal vein; ^*p < 0.05; ^**p < 0.01; ^***p < 0.001. See also Figure S2.

Expression Data

Genes:	DsRed EGFP mCherry myb
Fish:	WT nz10Tg; zf188Tg nz50Tg; zf188Tg nz50Tg; zf188Tg + MO9-runx1
Condition:	bacterial treatment: Salmonella enterica subsp. enterica serovar Typhimurium
Knockdown Reagent:	MO9-runx1
Anatomical Terms:	mesenchyme hematopoietic stem cell ventral wall of dorsal aorta hematopoietic multipotent progenitor cell ventral wall of dorsal aorta hematopoietic stem cell ventral wall of dorsal aorta neutrophil
Stage:	Day 4

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	nz10Tg; zf188Tg nz50Tg; zf188Tg nz50Tg; zf188Tg + MO9-runx1
Condition:	bacterial treatment: Salmonella enterica subsp. enterica serovar Typhimurium
Knockdown Reagent:	MO9-runx1
Observed In:	granulocyte differentiation mesenchyme hematopoietic multipotent progenitor cell mesenchyme hematopoietic stem cell ventral wall of dorsal aorta hematopoietic stem cell ventral wall of dorsal aorta neutrophil
Stage:	Day 4

Phenotype Detail

Acknowledgments

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