ZFIN ID: ZDB-FIG-120209-8
Hall et al., 2012 - Infection-Responsive Expansion of the Hematopoietic Stem and Progenitor Cell Compartment in Zebrafish Is Dependent upon Inducible Nitric Oxide. Cell Stem Cell   10(2):198-209 Full text @ Cell Stem Cell
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Condition:
Knockdown Reagent:
Anatomical Terms:
Stage: Day 4
PHENOTYPE:
Fish:
Condition:
Knockdown Reagent:
Observed In:
Stage: Day 4

Fig. 2

HSPC Compartment Expands in Response to Infection (A) Confocal imaging through the AGM of control MO- and Runx1 MO-injected Tg(lyz:DsRED)/Tg(runx1P2:EGFP) larvae following infection, compared with PBS controls at 2 dpi. (B) Expression of cmyb within the AGM of uninjected, PBS control and infected larvae at 2 dpi. (C) Quantification of cmyb-expressing cells within the AGM (as defined in schematic) of larvae shown in (B); arrowhead marks somite boundary that intersects cloaca. (D) Quantification of dual-labeled HSPCs (marked by asterisks) within the subaortic space of infected Tg(runx1P2:EGFP)/Tg(kdrl:nls-mCherry) larvae, compared with PBS controls at 1 and 2 dpi. All views, anterior to left. Numbers represent frequency of embryos or larvae with displayed phenotype. Scale bar, 50 μm in (A). Abbreviations: DA, dorsal aorta; PCV, posterior cardinal vein; *p < 0.05; **p < 0.01; ***p < 0.001. See also Figure S2.

Gene Expression Details
Gene Antibody Fish Conditions Stage Qualifier Anatomy Assay
DsRed nz50Tg; zf188Tg control Day 4 ventral wall of dorsal aorta neutrophil IFL
nz50Tg; zf188Tg bacterial treatment: Salmonella enterica subsp. enterica serovar Typhimurium Day 4 ventral wall of dorsal aorta neutrophil IFL
nz50Tg; zf188Tg + MO9-runx1 bacterial treatment: Salmonella enterica subsp. enterica serovar Typhimurium Day 4 Not Detected ventral wall of dorsal aorta neutrophil IFL
nz50Tg; zf188Tg + MO9-runx1 standard conditions Day 4 Not Detected ventral wall of dorsal aorta neutrophil IFL
EGFP nz10Tg; zf188Tg standard conditions Day 4 mesenchyme hematopoietic stem cell IFL
nz10Tg; zf188Tg bacterial treatment: Salmonella enterica subsp. enterica serovar Typhimurium Day 4 mesenchyme hematopoietic stem cell IFL
nz50Tg; zf188Tg control Day 4 ventral wall of dorsal aorta hematopoietic multipotent progenitor cell IFL
Day 4 ventral wall of dorsal aorta hematopoietic stem cell IFL
nz50Tg; zf188Tg bacterial treatment: Salmonella enterica subsp. enterica serovar Typhimurium Day 4 ventral wall of dorsal aorta hematopoietic multipotent progenitor cell IFL
Day 4 ventral wall of dorsal aorta hematopoietic stem cell IFL
nz50Tg; zf188Tg + MO9-runx1 bacterial treatment: Salmonella enterica subsp. enterica serovar Typhimurium Day 4 Not Detected ventral wall of dorsal aorta hematopoietic multipotent progenitor cell IFL
Day 4 Not Detected ventral wall of dorsal aorta hematopoietic stem cell IFL
nz50Tg; zf188Tg + MO9-runx1 standard conditions Day 4 Not Detected ventral wall of dorsal aorta hematopoietic multipotent progenitor cell IFL
Day 4 Not Detected ventral wall of dorsal aorta hematopoietic stem cell IFL
mCherry nz10Tg; zf188Tg standard conditions Day 4 mesenchyme hematopoietic stem cell IFL
nz10Tg; zf188Tg bacterial treatment: Salmonella enterica subsp. enterica serovar Typhimurium Day 4 mesenchyme hematopoietic stem cell IFL
myb WT standard conditions Day 4 ventral wall of dorsal aorta hematopoietic stem cell ISH
WT bacterial treatment: Salmonella enterica subsp. enterica serovar Typhimurium Day 4 ventral wall of dorsal aorta hematopoietic stem cell ISH
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
nz10Tg; zf188Tg bacterial treatment: Salmonella enterica subsp. enterica serovar Typhimurium Day 4 mesenchyme hematopoietic multipotent progenitor cell increased amount, abnormal
Day 4 mesenchyme hematopoietic stem cell increased amount, abnormal
nz50Tg; zf188Tg bacterial treatment: Salmonella enterica subsp. enterica serovar Typhimurium Day 4 granulocyte differentiation increased occurrence, abnormal
Day 4 ventral wall of dorsal aorta hematopoietic stem cell increased amount, abnormal
Day 4 ventral wall of dorsal aorta neutrophil increased amount, abnormal
nz50Tg; zf188Tg + MO9-runx1 bacterial treatment: Salmonella enterica subsp. enterica serovar Typhimurium Day 4 granulocyte differentiation decreased occurrence, abnormal
Day 4 ventral wall of dorsal aorta hematopoietic stem cell decreased amount, abnormal
Day 4 ventral wall of dorsal aorta neutrophil decreased amount, abnormal
nz50Tg; zf188Tg + MO9-runx1 standard conditions Day 4 granulocyte differentiation decreased occurrence, abnormal
Day 4 ventral wall of dorsal aorta hematopoietic stem cell decreased amount, abnormal
Day 4 ventral wall of dorsal aorta neutrophil decreased amount, abnormal
Acknowledgments:
ZFIN wishes to thank the journal Cell Stem Cell for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Cell Stem Cell