ZFIN ID: ZDB-FIG-120209-10
Hall et al., 2012 - Infection-Responsive Expansion of the Hematopoietic Stem and Progenitor Cell Compartment in Zebrafish Is Dependent upon Inducible Nitric Oxide. Cell Stem Cell   10(2):198-209 Full text @ Cell Stem Cell
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Condition:
Knockdown Reagent:
Anatomical Terms:
Stage Range: Pec-fin to Day 4
PHENOTYPE:
Fish:
Condition:
Observed In:
Stage: Day 4

Fig. 4

cebpβ Is Expressed within HSPCs in Response to Infection and Is Required for Demand-Driven Granulopoiesis (A) Expression of cebpβ within the AGM of infected larvae, compared with PBS controls at 12 hpi and 2 dpi. (B) Dual WMISH of cebpβ and runx1 within the AGM of infected larvae at 12 hpi. (C) Dual WMISH of cebpβ and lyz within the AGM of infected larvae at 2 dpi. (D) Control MO- and C/ebpβ MO-injected Tg(lyz:EGFP)/Tg(gata1:DsRED) larvae following infection, compared with PBS controls at 2 dpi. Numbers represent frequency of embryos or larvae with displayed phenotype. Scale bar, 100 μm in (D). All views, anterior to left. Abbreviations: DA, dorsal aorta; PCV, posterior cardinal vein. See also Figure S4.

Gene Expression Details
Gene Antibody Fish Conditions Stage Qualifier Anatomy Assay
cebpb WT standard conditions Pec-fin Not Detected ventral wall of dorsal aorta ISH
Day 4 Not Detected ventral wall of dorsal aorta ISH
WT bacterial treatment: Salmonella enterica subsp. enterica serovar Typhimurium Pec-fin ventral wall of dorsal aorta ISH
Pec-fin ventral wall of dorsal aorta hematopoietic stem cell ISH
Day 4 ventral wall of dorsal aorta ISH
Day 4 ventral wall of dorsal aorta neutrophil ISH
EGFP nz117Tg; sd2Tg standard conditions Day 4 neutrophil IFL
nz117Tg; sd2Tg bacterial treatment: Salmonella enterica subsp. enterica serovar Typhimurium Day 4 neutrophil IFL
nz117Tg; sd2Tg + MO1-cebpb standard conditions Day 4 neutrophil IFL
nz117Tg; sd2Tg + MO1-cebpb bacterial treatment: Salmonella enterica subsp. enterica serovar Typhimurium Day 4 neutrophil IFL
lyz WT bacterial treatment: Salmonella enterica subsp. enterica serovar Typhimurium Day 4 ventral wall of dorsal aorta neutrophil ISH
runx1 WT bacterial treatment: Salmonella enterica subsp. enterica serovar Typhimurium Pec-fin ventral wall of dorsal aorta hematopoietic stem cell ISH
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
nz117Tg; sd2Tg bacterial treatment: Salmonella enterica subsp. enterica serovar Typhimurium Day 4 neutrophil increased amount, abnormal
Acknowledgments:
ZFIN wishes to thank the journal Cell Stem Cell for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Cell Stem Cell