ZFIN ID: ZDB-FIG-120209-12
Hall et al., 2012 - Infection-Responsive Expansion of the Hematopoietic Stem and Progenitor Cell Compartment in Zebrafish Is Dependent upon Inducible Nitric Oxide. Cell Stem Cell   10(2):198-209 Full text @ Cell Stem Cell
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Condition:
Knockdown Reagents:
Anatomical Term:
Stage: Day 4

Fig. 6

Nos2a Functions Downstream of C/ebpβ during Demand-Driven Granulopoiesis (A) Expression of nos2a within the AGM of control MO- and C/ebpβ MO-injected larvae following infection, compared with PBS controls at 2 dpi. (B) Expression of cebpβ within the AGM of control MO- and Nos2a MO-injected larvae following infection, compared with PBS controls at 2 dpi. (C) Expression of nos2a at 22 hpf, following injection of 50 pg cebpβ mRNA. (D) Dual WMISH of cebpβ and nos2a and immunofluorescence detection of lyz:EGFP within a Tg(lyz:EGFP) embryo at 22 hpf, following injection of 50 pg cebpβ mRNA. (E-G) lyz:EGFP-expressing myeloid progenitors on the yolk of noninjected, cebpβ mRNA-injected, and cebpβ mRNA/Nos2a MO-injected Tg(lyz:EGFP) embryos at 22 hpf, respectively. (H) Quantification of lyz:EGFP-expressing cells on the yolk of embryos in (E)-(G). Numbers represent frequency of embryos or larvae with displayed phenotype. All views, anterior to left. Scale bar, 10 μm in (D). Abbreviations: n.s., not significant; ***p < 0.001. See also Figure S6.

Gene Expression Details
Gene Antibody Fish Conditions Stage Qualifier Anatomy Assay
cebpb WT standard conditions Day 4 Not Detected ventral wall of dorsal aorta ISH
WT bacterial treatment: Salmonella enterica subsp. enterica serovar Typhimurium Day 4 ventral wall of dorsal aorta ISH
WT + MO3-nos2a bacterial treatment: Salmonella enterica subsp. enterica serovar Typhimurium Day 4 ventral wall of dorsal aorta ISH
WT + MO3-nos2a standard conditions Day 4 Not Detected ventral wall of dorsal aorta ISH
nos2a WT standard conditions Day 4 Not Detected ventral wall of dorsal aorta ISH
WT bacterial treatment: Salmonella enterica subsp. enterica serovar Typhimurium Day 4 ventral wall of dorsal aorta ISH
WT + MO1-cebpb standard conditions Day 4 Not Detected ventral wall of dorsal aorta ISH
WT + MO1-cebpb bacterial treatment: Salmonella enterica subsp. enterica serovar Typhimurium Day 4 ventral wall of dorsal aorta ISH
Antibody Labeling Details No data available
Phenotype Details No data available
Acknowledgments:
ZFIN wishes to thank the journal Cell Stem Cell for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Cell Stem Cell