ZFIN ID: ZDB-FIG-120209-12
Hall et al., 2012 - Infection-Responsive Expansion of the Hematopoietic Stem and Progenitor Cell Compartment in Zebrafish Is Dependent upon Inducible Nitric Oxide. Cell Stem Cell   10(2):198-209 Full text @ Cell Stem Cell
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Condition:
Knockdown Reagents:
Anatomical Term:
Stage: Day 4

Fig. 6

Nos2a Functions Downstream of C/ebpβ during Demand-Driven Granulopoiesis (A) Expression of nos2a within the AGM of control MO- and C/ebpβ MO-injected larvae following infection, compared with PBS controls at 2 dpi. (B) Expression of cebpβ within the AGM of control MO- and Nos2a MO-injected larvae following infection, compared with PBS controls at 2 dpi. (C) Expression of nos2a at 22 hpf, following injection of 50 pg cebpβ mRNA. (D) Dual WMISH of cebpβ and nos2a and immunofluorescence detection of lyz:EGFP within a Tg(lyz:EGFP) embryo at 22 hpf, following injection of 50 pg cebpβ mRNA. (E-G) lyz:EGFP-expressing myeloid progenitors on the yolk of noninjected, cebpβ mRNA-injected, and cebpβ mRNA/Nos2a MO-injected Tg(lyz:EGFP) embryos at 22 hpf, respectively. (H) Quantification of lyz:EGFP-expressing cells on the yolk of embryos in (E)-(G). Numbers represent frequency of embryos or larvae with displayed phenotype. All views, anterior to left. Scale bar, 10 μm in (D). Abbreviations: n.s., not significant; ***p < 0.001. See also Figure S6.

Gene Expression Details
Gene Antibody Fish Conditions Stage Qualifier Anatomy Assay
cebpb WT standard conditions Day 4 Not Detected ventral wall of dorsal aorta ISH
WT bacterial treatment: Salmonella enterica subsp. enterica serovar Typhimurium Day 4 ventral wall of dorsal aorta ISH
WT + MO3-nos2a bacterial treatment: Salmonella enterica subsp. enterica serovar Typhimurium Day 4 ventral wall of dorsal aorta ISH
WT + MO3-nos2a standard conditions Day 4 Not Detected ventral wall of dorsal aorta ISH
nos2a WT standard conditions Day 4 Not Detected ventral wall of dorsal aorta ISH
WT bacterial treatment: Salmonella enterica subsp. enterica serovar Typhimurium Day 4 ventral wall of dorsal aorta ISH
WT + MO1-cebpb standard conditions Day 4 Not Detected ventral wall of dorsal aorta ISH
WT + MO1-cebpb bacterial treatment: Salmonella enterica subsp. enterica serovar Typhimurium Day 4 ventral wall of dorsal aorta ISH
Antibody Labeling Details No data available
Phenotype Details No data available
Acknowledgments:
This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Cell Stem Cell