ZFIN ID: ZDB-FIG-120209-13
Hall et al., 2012 - Infection-Responsive Expansion of the Hematopoietic Stem and Progenitor Cell Compartment in Zebrafish Is Dependent upon Inducible Nitric Oxide. Cell Stem Cell   10(2):198-209 Full text @ Cell Stem Cell
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Condition:
Anatomical Terms:
Stage Range: Day 5 to Days 7-13
PHENOTYPE:
Fish:
Condition:
Observed In:
Stage Range: Day 4 to Days 7-13

Fig. S1

(A) Time-lapse confocal imaging of neutrophil response to 300 cfu Sal-GFP injected into the hindbrain ventricle of Tg(lyz:DsRED) embryos at 50 hpf. (B) 3D reconstruction of A at 4 hpi; inset, magnified view of boxed neutrophil with cutting plane to reveal phagocytosed Sal-GFP. (C) Tg(lyz:DsRED) larvae 1 day following injection of 600 cfu Sal-GFP demonstrating either diminished neutrophil numbers or extensive bacterial burden, compared to PBS control. (D) Survival and demand-driven granulopoiesis frequency relative to infection dose. Percent survival (in black) relative to hindbrain injection dose (cfu) and % of surviving infected larvae demonstrating demand-driven granulopoiesis (in red) relative to infection dose (solid lines represent mean with 95% confidence interval dashed) as scored at 2 dpi. Light blue box highlights infection dose range used in this study. (E) QPCR expression analysis of lyz, mpx, csf1ra, lcp1 and spi1 within infected larvae at 2.25 and 3.25 dpi, relative to PBS controls, means standard ± errors. (F and G) Infected larvae demonstrate reduced lymphopoiesis. (F) Expression and quantification of ikaros-expressing cells within the AGM of uninjected (4 dpf), PBS control and infected larvae at 2dpi. (G) Confocal imaging through the thymus of Tg(lyz:DsRED)/Tg(lck:GFP) infected larvae (compared with PBS controls). Volumes of lck:GFP-expressing cells within the thymus are represented as mean standard deviation. Thymus reconstructions are paired with images through the AGM region of same larvae. All views, anterior to left. *, p-value<0.05; **, p-value<0.01; ***, p-value<0.001. Scale bars, 50μm in B and F.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
DsRed cz2Tg; nz50Tg standard conditions Day 5 neutrophil IFL
Day 6 neutrophil IFL
Days 7-13 neutrophil IFL
cz2Tg; nz50Tg bacterial treatment: Salmonella enterica subsp. enterica serovar Typhimurium Day 5 neutrophil IFL
Day 6 neutrophil IFL
Days 7-13 neutrophil IFL
EGFP cz2Tg; nz50Tg standard conditions Day 5 thymus cell IFL
Day 6 thymus cell IFL
Days 7-13 thymus cell IFL
cz2Tg; nz50Tg bacterial treatment: Salmonella enterica subsp. enterica serovar Typhimurium Day 5 thymus cell IFL
Day 6 thymus cell IFL
Days 7-13 thymus cell IFL
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
WT bacterial treatment: Salmonella enterica subsp. enterica serovar Typhimurium Day 4 thymus cell decreased amount, abnormal
cz2Tg; nz50Tg bacterial treatment: Salmonella enterica subsp. enterica serovar Typhimurium Day 5 thymus cell decreased amount, abnormal
Day 6 thymus cell decreased amount, abnormal
Days 7-13 thymus cell decreased amount, abnormal
Acknowledgments:
ZFIN wishes to thank the journal Cell Stem Cell for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Cell Stem Cell