FIGURE

Fig. 1

ID

ZDB-FIG-120201-11

Publication

Dworkin et al., 2012 - Midbrain-hindbrain boundary patterning and morphogenesis are regulated by diverse grainy head-like 2-dependent pathways

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Fig. 1

Loss of function of grhl2b leads to severe defects in the MHB region. (A,B) Embryos injected with control or grhl2b ATG-blocking MO. Lateral views with dorsal views in the insets. MB, midbrain; HB, hindbrain; apoptosis and folding defects are indicated by arrows and arrowheads. (C,D) Apoptosis is increased in grhl2b morphants (MO:grhl2b) compared with controls. (E-H) Positioning of either the midbrain (otx2) or hindbrain (gbx1) territories within the neural plate in grhl2b morphants compared with controls. (I-L) Expression of eng2a in grhl2b morphants and controls from the onset of MHB formation. (M-V) Expression of MHB patterning genes (eng2a, her5, wnt1 and pax2a) at 26 hpf in grhl2b morphants and controls. mhb, midbrain-hindbrain boundary; os, optic stalk; ov, otic vesicles.

Expression Data

Genes:	en2a gbx1 her5 otx2b pax2a wnt1
Fish:	WT WT + MO2-grhl2b
Knockdown Reagent:	MO2-grhl2b
Anatomical Terms:	midbrain hindbrain boundary midbrain hindbrain boundary neural tube presumptive neural plate
Stage Range:	Shield to Prim-5

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	WT + MO2-grhl2b
Knockdown Reagent:	MO2-grhl2b
Observed In:	hindbrain midbrain hindbrain boundary midbrain-hindbrain boundary morphogenesis ventricular system ventricular system development whole organism
Stage Range:	1-cell to Prim-5

Phenotype Detail

Acknowledgments

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