FIGURE
Fig. S2
- ID
- ZDB-FIG-120201-18
- Publication
- Dworkin et al., 2012 - Midbrain-hindbrain boundary patterning and morphogenesis are regulated by diverse grainy head-like 2-dependent pathways
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Fig. S2
Generation, characterisation and effects of a grhl2b exon 1/intron 1 splice-blocking morpholino. (A) Schematic of PCR primers used to detect non-spliced reverse-transcribed mRNA product following block of splicing of grhl2b. (B) A specific grhl2b non-spliced product was detectable by RT-PCR; this product was absent in MO:control-injected fish. (C,D) The grhl2b splice-blocking MO reproduced the MHB-folding (C) and apoptosis (TUNEL; D) phenotype observed with the grhl2b ATG-blocking MO. The apoptosis in grhl2b morphants is most abundant in, but not restricted to, neural regions. |
Expression Data
Expression Detail
Antibody Labeling
Phenotype Data
| Fish: | |
|---|---|
| Knockdown Reagent: | |
| Observed In: | |
| Stage: | Prim-5 |
Phenotype Detail
Acknowledgments
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