FIGURE

Fig. S1

ID

ZDB-FIG-120201-17

Publication

Dworkin et al., 2012 - Midbrain-hindbrain boundary patterning and morphogenesis are regulated by diverse grainy head-like 2-dependent pathways

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Fig. S1

Comparison of human, mouse and zebrafish Grhl2 orthologs. (A) Amino acid alignment of Grhl2 from human (hGrhl2), mouse (mGrhl2) and zebrafish (zGrhl2a;zGrhl2b); boxed regions highlight highly conserved functional domains (TA, transactivation domain; DBD, DNA-binding domain; DIM, dimerisation domain). (B) Table showing the percentage identity and homology between human, mouse and zebrafish grhl2a and grhl2b sequences at the amino acid level. (C-G) Analysis of zebrafish grhl2b mRNA expression by in situ hybridisation from 8 hpf to 24 hpf. p, polster; anp and lnp, anterior and lateral neural plate; bp, brain primordium; nc, neural crest, ov, presumptive otic vesicles; op, optic primordium; oe, olfactory epithelium; pm, pronephric mesoderm. Expression is seen in the neural crest, both adaxially and ventrally to the neural tube (inset, nt, sagittal view) and otic vesicles. Flat-mount, dorsal view at 24 hpf (G) shows persistent expression in the olfactory epithelium, otic vesicles and neural crest.

Expression Data

Gene:	grhl2b
Fish:	WT
Anatomical Terms:	anterior axial hypoblast anterior neural plate anterior neural rod neural crest neural tube optic vesicle otic placode otic vesicle polster primitive olfactory epithelium pronephric duct posterior region (all 11)
Stage Range:	75%-epiboly to Prim-5

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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