ZFIN ID: ZDB-FIG-111102-14
Gore et al., 2011 - Rspo1/Wnt signaling promotes angiogenesis via Vegfc/Vegfr3. Development (Cambridge, England)   138(22):4875-4886 Full text @ Development
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Anatomical Terms:
Stage Range: 20-25 somites to Prim-5
PHENOTYPE:
Fish:
Observed In:
Stage Range: 20-25 somites to Prim-5

Fig. 5

Expression of Vegfc is specifically affected in dtty135 mutant embryos. (A-F) In situ hybridization of the mid-trunk of wild-type sibling (A,C,E) and dtty135 mutant (B,D,F) zebrafish embryos probed for vegfaa (A,B), vegfab (C,D) or vegfr3 (E,F). The expression of vegfaa in the somites (arrow in B), vegfab in the axial vessels (arrow in D) and vegfr3 in the axial vessels (arrows in F) does not change in dtty135 mutant embryos compared with their wild-type siblings. (A-D) 24 hpf; (E,F) 20 hpf. (G,H) In situ hybridization of 26 hpf wild-type sibling (G) and dtty135 mutant (H) embryos probed for vegfc (arrows). (I,K) In situ hybridization of 26 hpf wild-type embryos probed for vegfc, showing expression in the DA and ISV (K shows a magnified image of the boxed region in I). (J,L) In situ hybridization of 26 hpf wild-type embryos probed for vegfr3, showing expression in the CV and ISV tip cells (L shows a magnified image of the boxed region shown in J). (M) Double in situ hybridization confirming expression of Vegfr3/flt4 (purple) in the tip cell (arrows) in addition to the cardinal vein (arrowheads), when compared with expression of Vegfr2/flk1 (pink) throughout the DA, CV and ISV. Scale bars: 50 μm in B,D,F,G; 45 μm in I,J; 25 μm in K-M.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
flt4 rspo1y135/y135; y1Tg/y1Tg standard conditions 20-25 somites vascular cord ISH
y1Tg/y1Tg standard conditions 20-25 somites vascular cord ISH
Prim-5 axial vasculature ISH
Prim-5 caudal vein plexus ISH
Prim-5 intersegmental vessel endothelial tip cell ISH
kdrl y1Tg/y1Tg standard conditions Prim-5 caudal vein plexus ISH
Prim-5 dorsal aorta ISH
Prim-5 intersegmental vessel ISH
vegfaa rspo1y135/y135; y1Tg/y1Tg standard conditions Prim-5 axial vasculature ISH
y1Tg/y1Tg standard conditions Prim-5 axial vasculature ISH
vegfab rspo1y135/y135; y1Tg/y1Tg standard conditions Prim-5 axial vasculature ISH
y1Tg/y1Tg standard conditions Prim-5 axial vasculature ISH
vegfc rspo1y135/y135; y1Tg/y1Tg standard conditions Prim-5 axial vasculature ISH
y1Tg/y1Tg standard conditions Prim-5 axial vasculature ISH
Prim-5 dorsal aorta ISH
Prim-5 intersegmental vessel ISH
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
rspo1y135/y135; y1Tg/y1Tg standard conditions 20-25 somites axial blood vessel morphology, normal
Prim-5 axial blood vessel morphology, normal
Acknowledgments:
ZFIN wishes to thank the journal Development (Cambridge, England) for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Development