ZFIN ID: ZDB-FIG-111102-10
Gore et al., 2011 - Rspo1/Wnt signaling promotes angiogenesis via Vegfc/Vegfr3. Development (Cambridge, England)   138(22):4875-4886 Full text @ Development
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Anatomical Terms:
Stage Range: Prim-5 to Long-pec
PHENOTYPE:
Fish:
Observed In:
Stage Range: 20-25 somites to Long-pec

Fig. 1

dtty135 mutants have defects in angiogenesis, but not in endothelial specification or in vasculogenesis. (A) Diagram of a zebrafish embryo with the red box highlighting the region shown in B and C, and a blue box highlighting the region shown in D-G. (B,C) Confocal images of trunk vessels in 26 hpf Tg(fli-EGFP)y1 wild-type sibling (B) and dtty135 mutant (C) zebrafish, showing normal formation of the vasculogenic dorsal aorta and posterior cardinal vein but failure to form the angiogenic intersegmental vessels (arrows in B, asterisks show absence in C) in dtty135 mutants (lateral views, anterior towards the left). (D,E) The hindbrain vessels imaged in F,G. Primordial hindbrain channels (PHBC) are in blue, basilar artery (BA) is in red and the central arteries (CA) are in black. (F,G) Confocal images of hindbrain vessels in 48 hpf Tg(fli-EGFP)y1 wild-type sibling (F) and dtty135 mutant (G) zebrafish, showing normal formation of vasculogenic primary vessels, including the PHBC and BA, but failure to form the angiogenic CA that penetrate the hindbrain (dorsal views, anterior towards the left). (H,I) In situ hybridization of endothelium in the trunk of 26 hpf wild-type sibling (H) and dtty135 mutant (I) zebrafish with a probe for vecdn, showing normal expression levels in dtty135 mutants (lateral views, anterior towards the left). ISV are seen in wild-type siblings (arrows in H) but not in dtty135 mutants (asterisks in I). (J,K) Transmitted light images of 26 hpf Tg(fli-EGFP)y1 wild-type sibling (J) and dtty135 mutant (K) zebrafish, showing reduced width of yolk extension but otherwise normal morphology of dtty135 mutants (J) compared with their wild-type siblings (K). Images are lateral views, anterior towards the left. Endothelial proliferation is reduced in dtty135 mutants. (L) Total number of endothelial cells present in the three posteriormost trunk segments, measured in Tg(fli-nEGFP)y7 transgenic dtty135 mutant or wild-type sibling animals, at 26 hpf and 40 hpf. (M) Total number of phosphohistone H3-positive endothelial cells present in the three posteriormost trunk segments, measured in phosphohistone and GFP antibody-probed Tg(fli-nEGFP)y7 transgenic dtty135 mutant or wild-type sibling animals, at 26 hpf and 40 hpf. Data are meanĀ±s.e.m. (N,O) Higher-magnification confocal images of ISV sprouts in wild-type sibling (N) and dtty135 mutant (O) embryos at 20 hpf, showing reduced filopodial protrusions in mutants. (P) Total number of filopodia formed by control and mutant sprouts. Scale bars: 50 μm in B,C,F,G; 100 μm in H-K; 20 μm in N,O.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
cdh5 rspo1y135/y135; y1Tg/y1Tg standard conditions Prim-5 axial vasculature ISH
y1Tg/y1Tg standard conditions Prim-5 axial vasculature ISH
Prim-5 intersegmental vessel ISH
EGFP y1Tg/y1Tg standard conditions Prim-5 vasculature IFL
Long-pec vasculature IFL
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
rspo1y135/y135; y1Tg/y1Tg standard conditions 20-25 somites angiogenesis disrupted, abnormal
20-25 somites vascular sprouts filopodium absent, abnormal
Prim-5 angiogenesis disrupted, abnormal
Prim-5 extension decreased width, abnormal
Prim-5 intersegmental vessel absent, abnormal
Prim-25 angiogenesis disrupted, abnormal
Prim-25 trunk endothelial cell decreased amount, abnormal
Long-pec angiogenesis disrupted, abnormal
Long-pec anterior mesencephalic central artery absent, abnormal
Long-pec cerebellar central artery absent, abnormal
Long-pec intersegmental vessel decreased length, abnormal
Long-pec middle mesencephalic central artery absent, abnormal
Long-pec posterior mesencephalic central artery absent, abnormal
Long-pec trunk lymph vasculature absent, abnormal
Acknowledgments:
ZFIN wishes to thank the journal Development (Cambridge, England) for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Development