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ZIRC
ZFIN ID: ZDB-FIG-111102-17
Gore et al., 2011 - Rspo1/Wnt signaling promotes angiogenesis via Vegfc/Vegfr3. Development (Cambridge, England)   138(22):4875-4886 Full text @ Development
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Antibodies:
Fish:
Anatomical Terms:
Stage Range: 14-19 somites to Prim-5

Fig. S1

Endothelial, hematopoietic and muscle patterning is normal in dtty135 mutants. (A-J) In situ hybridization of the trunks of wild-type (A,C,E,G,I) and dtty135 mutant (B,D,F,H,J) zebrafish embryos probed for flk1 (A,B), dll4 (C,D), grl (E,F), dab2 (G,H) and gata1 (I,J). Marker expression appears equivalent in wild-type and dtty135 mutant animals. Embryos are at 26 hpf (A-H) and 18 hpf (I,J). (K,L) DIC transmitted light images of the trunks of 26 hpf wild-type (K) and dtty135 mutant (L) animals, showing normally patterned somites and muscles in dtty135 mutants. (M,N) Confocal imaging of trunks of wild-type (M) and dtty135 mutant (N) animals stained for immunofluorescence with a monoclonal anti-engrailed antibody (4D9), revealing normal muscle pioneer specification. (O,P) Confocal imaging of trunks of wild-type (O) and dtty135 mutant (P) animals stained for immunofluorescence with a monoclonal anti-SMMHC antibody (F59), revealing normal slow muscle differentiation. (Q,R) DIC transmitted light images of the trunks of 96 hpf wild-type (Q) and dtty135 mutant (R) animals, showing reduced muscle mass by 96 hpf in dtty135 mutants. Lateral views, anterior towards the left.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
dab2 rspo1y135/y135; y1Tg/y1Tg standard conditions Prim-5 vasculature ISH
y1Tg/y1Tg standard conditions Prim-5 vasculature ISH
dll4 rspo1y135/y135; y1Tg/y1Tg standard conditions Prim-5 vasculature ISH
y1Tg/y1Tg standard conditions Prim-5 vasculature ISH
gata1a rspo1y135/y135; y1Tg/y1Tg standard conditions 14-19 somites intermediate cell mass of mesoderm ISH
y1Tg/y1Tg standard conditions 14-19 somites intermediate cell mass of mesoderm ISH
hey2 rspo1y135/y135; y1Tg/y1Tg standard conditions Prim-5 vasculature ISH
y1Tg/y1Tg standard conditions Prim-5 vasculature ISH
kdrl rspo1y135/y135; y1Tg/y1Tg standard conditions Prim-5 vasculature ISH
y1Tg/y1Tg standard conditions Prim-5 vasculature ISH
Antibody Labeling Details
Antibody Assay Fish Conditions Stage Anatomy
Ab-F59 IHC rspo1y135/y135; y1Tg/y1Tg standard conditions Prim-5 slow muscle cell
IHC y1Tg/y1Tg standard conditions Prim-5 slow muscle cell
Ab1-eng IHC rspo1y135/y135; y1Tg/y1Tg standard conditions Prim-5 muscle pioneer
IHC y1Tg/y1Tg standard conditions Prim-5 muscle pioneer
Phenotype Details No data available
Acknowledgments:
ZFIN wishes to thank the journal Development (Cambridge, England) for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Development