header logo image header logo text
Downloads Login
Research
General Information
ZIRC
ZFIN ID: ZDB-FIG-111102-12
Gore et al., 2011 - Rspo1/Wnt signaling promotes angiogenesis via Vegfc/Vegfr3. Development (Cambridge, England)   138(22):4875-4886 Full text @ Development
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Anatomical Terms:
Stage: Prim-5
PHENOTYPE:
Fish:
Knockdown Reagents:
Observed In:
Stage: Prim-5

Fig. 3

Canonical Wnt signaling is required endothelial cell-autonomously for angiogenesis. (A) Diagram of a zebrafish embryo with the red box highlighting the region shown in B-F,H-J. (B) In situ hybridization of the trunk of a 26 hpf wild-type zebrafish embryo probed for wnt2bb, showing expression in the axial vessels (dorsal aorta, arrow; posterior cardinal vein, arrowhead). Lateral view, anterior towards the left. (C-F) Confocal images of trunk vessels in 26 hpf Tg(fli-EGFP)y1 wild-type zebrafish injected with either 5 ng control MO (C), 5 ng wnt2bb MO (D), 5 ng rspo1 MO (E) or 2.5 ng wnt2bb MO + 2.5 ng rspo1 MO (F). (G) Quantitation of the intersegmental vessel (ISV) phenotypes of morpholino-injected wild-type 26 hpf Tg(fli-EGFP)y1 zebrafish. (H-J) Confocal images of trunk vessels in 26 hpf wild-type Tg(flk-mCherry) zebrafish (red) injected with DNA for either control flk:EGFP (H) or flk:axin-EGFP (I,J) expression constructs (green), showing defects in sprouting (J) or growth (I) of ISV expressing the axin-EGFP fusion protein. (K) Quantitation of the intersegmental vessel (ISV) phenotypes of endothelium expressing flk:axin-EGFP or control flk:EGFP expression constructs. In G and K bars show the percentages of ISV that have failed to sprout (blue), ISV that have grown only up to the horizontal myoseptum half-way up the trunk (red) and ISV that have grown all the way to the dorsal trunk to form the DLAV (yellow). The number of segments counted is shown above each bar. Scale bars: 50 μm in B-F,H-J.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
EGFP y1Tg/y1Tg standard conditions Prim-5 vasculature IFL
wnt2bb y1Tg/y1Tg standard conditions Prim-5 axial vasculature ISH
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
y1Tg/y1Tg + MO1-wnt2bb standard conditions Prim-5 angiogenesis disrupted, abnormal
Prim-5 intersegmental vessel decreased length, abnormal
y1Tg/y1Tg + MO1-wnt2bb + MO3-rspo1 standard conditions Prim-5 angiogenesis disrupted, abnormal
Prim-5 intersegmental vessel absent, abnormal
y1Tg/y1Tg + MO3-rspo1 standard conditions Prim-5 angiogenesis disrupted, abnormal
Prim-5 intersegmental vessel decreased length, abnormal
Acknowledgments:
ZFIN wishes to thank the journal Development (Cambridge, England) for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Development