FIGURE

Fig. 5

ID

ZDB-FIG-110929-52

Publication

Wythe et al., 2011 - Hadp1, a newly identified pleckstrin homology domain protein, is required for cardiac contractility in zebrafish

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Fig. 5

Non-chamber myocardium fails to differentiate properly in hadp1 morphants. (A–J) Frontal views at 48 hpf (head to the top) illustrate expression of chamber-specific genes in control and hadp1 morphant embryos. (A,B) atrial myosin heavy chain (amhc; red; atrial marker) and ventricular myosin heavy chain (vmhc; purple; ventricular marker) were visualized by double in situ hybridization. (C,D) MF20 (red) and S46 (green) were visualized by whole mount immunofluorescence. (E,F) bmp4 expression was examined by in situ hybridization. Ventricle (V), atrium (A) and outflow tract (OFT) are indicated. Arrows indicate the AV boundary, and the dotted line demarcates the cardiac silhouette. (G,H) Immunofluorescence was used to study cmlc2:GFP (green) and DM-GRASP (red) expression. Arrows and arrowheads indicate myocardium and endocardium, respectively. (I,J) Confocal analysis of the AV canal of control (I) and hadp1 morphant (J) hearts shown in G,H. The superior (sup) and inferior (inf) aspects of the canal are shown, and remodeled trapezoidal cells are indicated with asterisks, whereas non-remodeled columnar cardiomyocytes are indicated with plus signs. Arrows and arrowheads indicate myocardium and endocardium, respectively. (K,L) notch1b expression was examined by in situ hybridization. Ventricle (V) and atrium (A) are indicated. Arrows indicate the AV boundary.

Expression Data

Genes:	bmp4 EGFP myh6 myh7 notch1b
Antibodies:	Ab-MF20 Ab-S46 zn-8
Fish:	AB AB + MO1-plekha7a twu34Tg twu34Tg + MO1-plekha7a
Knockdown Reagent:	MO1-plekha7a
Anatomical Terms:	atrial myocardium atrioventricular canal myocardium atrioventricular canal endocardium atrium cardiac ventricle myocardium
Stage:	Long-pec

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	twu34Tg + MO1-plekha7a
Knockdown Reagent:	MO1-plekha7a
Observed In:	atrial ventricular junction remodeling atrioventricular canal cardiac muscle cell
Stage:	Long-pec

Phenotype Detail

Acknowledgments

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