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ZIRC
ZFIN ID: ZDB-ATB-081006-1
Antibody Name: Ab-MF20
Synonyms: anti-MyHC (1), MF 20 (1), MF-20 (1), MF20 (1), anti-meromyosin (1), anti-myosin heavy chain monoclonal MF20 (1)

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(Including Attributions)
Host Organism: Mouse
Immunogen Organism: Chicken
Isotype: IgG2b
Type: monoclonal
Assays: Immunohistochemistry, Western blot
Antigen Genes:
Source: Developmental Studies Hybridoma Bank
Wiki:
Note: Production of this antibody was first described in Bader et al. (1982) JCB 95:763-770.
NOTES
Comment Citation
Labels both fast and slow sarcomeric MyHCs, binds to an epitope in the myosin  ... ZFIN Curated Data
labels slow and fast muscle fibers. Barresi et al., 2001
ANATOMICAL LABELING
Anatomy Stage Assay Gene Data
abductor hyohyoid Day 5 IHC Fig. 1 with image  from  Knight et al., 2011
adductor hyohyoid Day 4 IHC Fig. 3 with image  from  Wiweger et al., 2014
Day 5 IHC Fig. 1 with image  from  Knight et al., 2011
adductor hyomandibulae Protruding-mouth IHC Fig. 1 with image  from  Guner-Ataman et al., 2018
Day 5 IHC Fig. 1 with image  from  Knight et al., 2011
adductor mandibulae Long-pec IHC Fig. 2 with image  from  van der Velden et al., 2013
Pec-fin IHC Fig. 2 with image  from  van der Velden et al., 2013
Protruding-mouth IHC 6 figures with image  from  4 publications
Day 4 IHC 2 figures with image  from  2 publications
adductor operculi Protruding-mouth IHC 4 figures with image  from  3 publications
Day 4 IHC 2 figures with image  from  2 publications
Day 5 IHC Fig. 1 with image  from  Knight et al., 2011
atrial myocardium Long-pec IHC 5 figures with image  from  3 publications
atrium Long-pec IHC 8 figures with image  from  6 publications
Protruding-mouth IHC 3 figures with image  from  2 publications
atrium cardiac muscle cell Long-pec IHC Fig. 5  from  van't Padje et al., 2009
atrium myosin complex Protruding-mouth IHC Fig. 3  from  Meder et al., 2010
branchial muscle Protruding-mouth IHC Fig. 5 with image  from  Dupret et al., 2016
bulbus arteriosus Long-pec IHC 3 figures with image  from  Zeng et al., 2014
cardiac muscle cell 20-25 somites IHC Fig. 1 with image  from  George et al., 2015
Prim-5 IHC Fig. 1  from  Zhou et al., 2011
Prim-25 IHC Fig. 2  from  Yan et al., 2013
Long-pec IHC 3 figures with image  from  3 publications
Pec-fin IHC 3 figures  from  2 publications
Protruding-mouth IHC 3 figures with image  from  3 publications
Day 4 IHC 2 figures with image  from  Uribe et al., 2018
Day 5 IHC Fig. 5 with image  from  Uribe et al., 2018
cardiac muscle cell A band Protruding-mouth IHC Fig. 1  from  Hassel et al., 2009
cardiac ventricle Long-pec IHC 12 figures with image  from  10 publications
Protruding-mouth IHC 5 figures with image  from  4 publications
cardiac ventricle cardiac muscle cell Long-pec IHC Fig. 5  from  van't Padje et al., 2009
Protruding-mouth IHC Fig. 8  from  Clemen et al., 2010
cardiac ventricle myosin complex Protruding-mouth IHC Fig. 3  from  Meder et al., 2010
cardiac ventricle sarcomere Long-pec IHC Fig. S2 with image  from  Samson et al., 2013
dilatator operculi Protruding-mouth IHC 4 figures with image  from  3 publications
Day 4 IHC 2 figures with image  from  2 publications
Day 5 IHC Fig. 1 with image  from  Knight et al., 2011
esophageal sphincter Protruding-mouth IHC Fig. 3 with image  from  Minchin et al., 2013
Day 4 IHC Fig. 3 with image  from  Minchin et al., 2013
esophagus skeletal muscle cell Protruding-mouth IHC Fig. 3 with image  from  Minchin et al., 2013
Day 4 IHC 2 figures with image  from  Minchin et al., 2013
extraocular musculature Protruding-mouth IHC Fig. 1 with image  from  Matsui et al., 2015
eye muscle Protruding-mouth IHC Fig. 5 with image  from  Dupret et al., 2016
fast muscle cell Prim-5 IHC 3 figures with image  from  2 publications
Long-pec IHC 2 figures  from  Hirata et al., 2012
head muscle Protruding-mouth IHC Fig. 5 with image  from  Dupret et al., 2016
Day 6 IHC Fig. 5 with image  from  Dupret et al., 2016
heart Long-pec IHC 11 figures with image  from  10 publications
Protruding-mouth IHC Fig. 8  from  Gupta et al., 2012
Day 4 IHC Fig. 5  from  Guan et al., 2013
heart myofibril Protruding-mouth IHC Fig. 6  from  Glenn et al., 2012
Day 5 IHC Fig. 6  from  Glenn et al., 2012
heart myosin complex Long-pec IHC Fig. 1  from  Gao et al., 2010
heart myosin filament Long-pec IHC Fig. 6 with image  from  Lu et al., 2011
heart sarcomere Long-pec IHC Fig. S2 with image  from  Samson et al., 2013
heart tube Prim-5 IHC 2 figures with image  from  2 publications
Prim-15 IHC 2 figures with image  from  Zeng et al., 2014
Prim-25 IHC Fig. 1 with image  from  Yelon et al., 2000
heart tube cardiac muscle cell Prim-5 IHC 2 figures  from  Zhou et al., 2011
heart tube myosin filament Prim-5 IHC Fig. 6 with image  from  Lu et al., 2011
Prim-15 IHC Fig. 6 with image  from  Lu et al., 2011
hyohyoideus Pec-fin IHC Fig. 2 with image  from  van der Velden et al., 2013
Protruding-mouth IHC 6 figures with image  from  4 publications
Day 4 IHC 2 figures with image  from  2 publications
hyoid muscle Protruding-mouth IHC Fig. 4 with image  from  Knight et al., 2011
inferior hyohyoid Day 5 IHC Fig. 1 with image  from  Knight et al., 2011
infracarinalis Day 4 IHC Fig. 5 with image  from  Elworthy et al., 2008
interhyoideus Pec-fin IHC Fig. 2 with image  from  van der Velden et al., 2013
Protruding-mouth IHC 6 figures with image  from  4 publications
Day 4 IHC 2 figures with image  from  2 publications
levator arcus palatini Protruding-mouth IHC 4 figures with image  from  3 publications
Day 4 IHC 2 figures with image  from  2 publications
Day 5 IHC Fig. 1 with image  from  Knight et al., 2011
levator operculi Protruding-mouth IHC 3 figures with image  from  3 publications
Day 5 IHC Fig. 1 with image  from  Knight et al., 2011
mandibular muscle Protruding-mouth IHC Fig. 4 with image  from  Knight et al., 2011
mesoderm Prim-5 IHC Fig. 3 with image  from  Araya et al., 2014
muscle Prim-5 IHC 2 figures with image  from  2 publications
Day 4 IHC 2 figures with image  from  Elworthy et al., 2008
muscle cell 10-13 somites IHC Fig. 2 with image  from  Windner et al., 2012
14-19 somites IHC Fig. 2 with image  from  Windner et al., 2012
20-25 somites IHC Fig. 2 with image  from  Windner et al., 2012
Prim-5 IHC 3 figures with image  from  2 publications
Long-pec IHC Fig. 6 with image  from  Taglietti et al., 2016
myocardium Prim-5 IHC Fig. S2 with image  from  Zeng et al., 2014
Prim-15 IHC 2 figures with image  from  2 publications
Prim-25 IHC Fig. 4 with image  from  Zeng et al., 2014
Long-pec IHC 4 figures with image  from  4 publications
Protruding-mouth IHC 2 figures with image  from  2 publications
Day 4 IHC Fig. 2 with image  from  Singleman et al., 2012
Day 5 IHC Fig. 2 with image  from  Singleman et al., 2012
Days 14-20 IHC Fig. 6 with image  from  Cheng et al., 2016
Days 30-44 IHC Fig. 3 with image  from  Cheng et al., 2016
Days 45-89 IHC Fig. 6 with image  from  Cheng et al., 2016
myotome 26+ somites IHC Fig. 3 with image  from  Windner et al., 2015
Prim-5 IHC Fig. 2 with image  from  Windner et al., 2015
Prim-25 IHC 4 figures with image  from  2 publications
myotome fast muscle cell Prim-5 IHC 2 figures with image  from  Naganawa et al., 2011
Long-pec IHC 2 figures with image  from  Naganawa et al., 2011
myotome skeletal muscle cell Long-pec IHC Fig. 2  from  Völkers et al., 2012
Protruding-mouth IHC Fig. 2  from  Völkers et al., 2012
pectoral fin Protruding-mouth IHC Fig. 13  from  Nogueira et al., 2015
pectoral fin musculature Protruding-mouth IHC Fig. 5 with image  from  Dupret et al., 2016
Day 4 IHC Fig. 6 with image  from  Banerjee et al., 2015
pharyngeal musculature Protruding-mouth IHC Fig. 1 with image  from  Matsui et al., 2015
Day 4 IHC Fig. 5 with image  from  Comyn et al., 2012
Day 5 IHC Fig. 4 with image  from  Knight et al., 2011
presumptive atrium heart tube Prim-25 IHC Fig. 4 with image  from  Zhu et al., 2017
presumptive bulbus arteriosus Prim-25 IHC Fig. 3 with image  from  Schindler et al., 2014
presumptive cardiac ventricle heart tube Prim-25 IHC 4 figures with image  from  2 publications
primitive heart tube 26+ somites IHC Fig. 5 with image  from  Devakanmalai et al., 2013
skeletal muscle 10-13 somites IHC Fig. 2  from  Chen et al., 2018
skeletal muscle cell Prim-5 IHC Fig. 4  from  Li et al., 2009
Long-pec IHC Fig. 3 with image  from  Machuca-Tzili et al., 2011
skeletal muscle cell A band Protruding-mouth IHC Fig. 1  from  Hassel et al., 2009
slow muscle cell 10-13 somites IHC Fig. 1 with image  from  Windner et al., 2015
Prim-5 IHC Fig. 4 with image  from  Lobbardi et al., 2011
slow muscle myoblast 5-9 somites IHC Fig. 4 with image  from  Windner et al., 2015
somite 14-19 somites IHC Fig. 5 with image  from  Jiang et al., 1996
Long-pec IHC Fig. 1 with image  from  Kimelman, 2016
sternohyoid Pec-fin IHC Fig. 2 with image  from  van der Velden et al., 2013
Protruding-mouth IHC 3 figures with image  from  3 publications
Day 4 IHC 2 figures with image  from  2 publications
Day 5 IHC Fig. 4 with image  from  Minchin et al., 2013
striated muscle cell Protruding-mouth IHC Fig. 4 with image  from  Jahangiri et al., 2016
supracarinalis Day 4 IHC Fig. 5 with image  from  Elworthy et al., 2008
ventral intermandibularis anterior Protruding-mouth IHC 6 figures with image  from  4 publications
Day 4 IHC 2 figures with image  from  2 publications
ventral intermandibularis posterior Protruding-mouth IHC 6 figures with image  from  4 publications
Day 4 IHC 2 figures with image  from  2 publications
ventricular myocardium Long-pec IHC 10 figures with image  from  6 publications
Protruding-mouth IHC Fig. 1  from  Völkers et al., 2012
Adult IHC Fig. 8 with image  from  George et al., 2015
whole organism Prim-5 WB 2 figures  from  2 publications
Long-pec WB 2 figures  from  2 publications
CITATIONS (252)