ZFIN ID: ZDB-FIG-110214-2
Radosevic et al., 2011 - Her9 represses neurogenic fate downstream of Tbx1 and retinoic acid signaling in the inner ear. Development (Cambridge, England)   138(3):397-408 Full text @ Development
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Knockdown Reagent:
Anatomical Terms:
Stage: Prim-5

Fig. 2 Blocking her9 function results in ectopic expression of the proneural genes neurod and neurod4. (A-J) In situ hybridization of 24 hpf wild-type (wt) and Her9 morphant zebrafish embryos to detect changes in neurog1 (A-D′), neurod (E-H′) and neurod4 (I,J) expression in the inner ear. (C-D′,G-H′) Double in situ hybridization for tbx1 (red chromogen in C,D,G,H and red fluorescence in C′,D′,G′,H′) and neurog1 (blue) or neurod (blue). Dashed circles delineate the otic vesicle. Arrows indicate ectopic neurogenesis. (K,L) Expression of high levels of her9 at the hindbrain rhombomeric boundaries (arrowheads). (M,N) neurod4 expression is expanded in the hindbrain. (O,P) krox20 expression in rhombomeres 3 and 5 is unchanged in Her9 morphants. A-J, K-N and O,P are at the same magnification.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
egr2b AB standard conditions Prim-5 rhombomere 3 ISH
Prim-5 rhombomere 5 ISH
AB + MO2-her9 standard conditions Prim-5 rhombomere 3 ISH
Prim-5 rhombomere 5 ISH
her9 AB standard conditions Prim-5 hindbrain ISH
AB + MO2-her9 standard conditions Prim-5 hindbrain ISH
neurod1 AB standard conditions Prim-5 otic vesicle ISH
AB + MO2-her9 standard conditions Prim-5 hindbrain ISH
Prim-5 otic vesicle postero-lateral region ISH
neurod4 AB standard conditions Prim-5 hindbrain ISH
Prim-5 otic vesicle ISH
AB + MO2-her9 standard conditions Prim-5 otic vesicle postero-lateral region ISH
neurog1 AB standard conditions Prim-5 otic vesicle ISH
AB + MO2-her9 standard conditions Prim-5 otic vesicle ISH
tbx1 AB standard conditions Prim-5 otic vesicle ISH
AB + MO2-her9 standard conditions Prim-5 otic vesicle ISH
Antibody Labeling Details No data available
Phenotype Details No data available
Acknowledgments:
ZFIN wishes to thank the journal Development (Cambridge, England) for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Development