FIGURE

Fig. 2

ID

ZDB-FIG-110214-2

Publication

Radosevic et al., 2011 - Her9 represses neurogenic fate downstream of Tbx1 and retinoic acid signaling in the inner ear

Other Figures

All Figure Page

Back to All Figure Page

Fig. 2

Blocking her9 function results in ectopic expression of the proneural genes neurod and neurod4. (A-J) In situ hybridization of 24 hpf wild-type (wt) and Her9 morphant zebrafish embryos to detect changes in neurog1 (A-D′), neurod (E-H′) and neurod4 (I,J) expression in the inner ear. (C-D′,G-H′) Double in situ hybridization for tbx1 (red chromogen in C,D,G,H and red fluorescence in C′,D′,G′,H′) and neurog1 (blue) or neurod (blue). Dashed circles delineate the otic vesicle. Arrows indicate ectopic neurogenesis. (K,L) Expression of high levels of her9 at the hindbrain rhombomeric boundaries (arrowheads). (M,N) neurod4 expression is expanded in the hindbrain. (O,P) krox20 expression in rhombomeres 3 and 5 is unchanged in Her9 morphants. A-J, K-N and O,P are at the same magnification.

Expression Data

Genes:	egr2b her9 neurod1 neurod4 neurog1 tbx1
Fish:	AB AB + MO2-her9
Knockdown Reagent:	MO2-her9
Anatomical Terms:	hindbrain otic vesicle otic vesicle postero-lateral region rhombomere 3 rhombomere 5
Stage:	Prim-5

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Development