FIGURE

Fig. 5

ID

ZDB-FIG-110214-5

Publication

Radosevic et al., 2011 - Her9 represses neurogenic fate downstream of Tbx1 and retinoic acid signaling in the inner ear

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Fig. 5

tbx1 is genetically upstream of her9. (A-C) Expression of her9 in 18 hpf zebrafish embryos from heterozygotic vgo^tm208 crosses. (D-I) Double in situ hybridization for tbx1 (red) and neurog1 (blue, D-F) or neurod (blue, G-I) in 24 hpf embryos from heterozygotic vgo^tm208 crosses. Arrows point to ectopic proneural expression. Two examples of vgo^tm208 mutants are given for each gene in A-I. (J) Proportion of descendants from heterozygotic crosses showing a strong phenotype (complete abrogation of her9 expression, substantial expansion of neurog1 and neurod), mild phenotype or wild-type phenotype at 24 hpf. (K,L) Double in situ hybridization for tbx1 (red) and her9 (blue) shows ectopic expression of her9 (arrow) after injection of capped tbx1 mRNA. (M,N) Double in situ hybridization for tbx1 (red) and neurod (blue) at 24 hpf shows downregulation of neurod after overexpression of capped tbx1 mRNA. All images are at the same magnification.

Expression Data

Genes:	her9 neurod1 neurog1 tbx1
Fish:	tbx1^tm208/tm208 tbx1^tm208/+
Anatomical Terms:	otic vesicle otic vesicle postero-lateral region
Stage:	14-19 somites

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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