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ZIRC
ZFIN ID: ZDB-FIG-110214-3
Radosevic et al., 2011 - Her9 represses neurogenic fate downstream of Tbx1 and retinoic acid signaling in the inner ear. Development (Cambridge, England)   138(3):397-408 Full text @ Development
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Knockdown Reagent:
Anatomical Terms:
Stage Range: Prim-5 to Day 4

Fig. 3 Depletion of her9 distorts the development of the SAG but not the generation of hair cells. (A,B) Three-dimensional reconstruction of the SAG at 48 hpf visualized in wild-type and her9-MO-injected zebrafish embryos carrying the islet3:GFP transgene. Arrowheads point to SAG branches. (C,D) Expression of atoh1a in wild-type and Her9 morphant embryos at 24 hpf. (E,F) Sagittal sections of posterior macula showing GFP fluorescence under the control of brn3c (pou4f3) reporter in a wild-type and her9-MO-injected embryo. (G,H) Three-dimensional reconstruction of selected confocal sections showing the otic cristae in wild-type and her9-MO-injected embryos. GFP in adjacent neuromasts of the lateral line has been removed for clarity. C,D, E,F and G,H are at the same magnification. SAG, statoacoustic ganglion; am, anterior macula; ac, anterior crista; lc, lateral crista; pc, posterior crista.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
atoh1a AB standard conditions Prim-5 anterior macula ISH
AB + MO2-her9 standard conditions Prim-5 anterior macula ISH
GFP s356tTg standard conditions Long-pec posterior macula IFL
Day 4 anterior crista IFL
Day 4 lateral crista IFL
Day 4 posterior crista IFL
s356tTg + MO2-her9 standard conditions Long-pec posterior macula IFL
Day 4 anterior crista IFL
Day 4 lateral crista IFL
Day 4 posterior crista IFL
zc7Tg standard conditions Long-pec statoacoustic (VIII) ganglion IFL
zc7Tg + MO2-her9 standard conditions Long-pec statoacoustic (VIII) ganglion IFL
Antibody Labeling Details No data available
Phenotype Details No data available
Acknowledgments:
ZFIN wishes to thank the journal Development (Cambridge, England) for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Development