ZFIN ID: ZDB-FIG-110214-3
Radosevic et al., 2011 - Her9 represses neurogenic fate downstream of Tbx1 and retinoic acid signaling in the inner ear. Development (Cambridge, England)   138(3):397-408 Full text @ Development
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Knockdown Reagent:
Anatomical Terms:
Stage Range: Prim-5 to Day 4

Fig. 3 Depletion of her9 distorts the development of the SAG but not the generation of hair cells. (A,B) Three-dimensional reconstruction of the SAG at 48 hpf visualized in wild-type and her9-MO-injected zebrafish embryos carrying the islet3:GFP transgene. Arrowheads point to SAG branches. (C,D) Expression of atoh1a in wild-type and Her9 morphant embryos at 24 hpf. (E,F) Sagittal sections of posterior macula showing GFP fluorescence under the control of brn3c (pou4f3) reporter in a wild-type and her9-MO-injected embryo. (G,H) Three-dimensional reconstruction of selected confocal sections showing the otic cristae in wild-type and her9-MO-injected embryos. GFP in adjacent neuromasts of the lateral line has been removed for clarity. C,D, E,F and G,H are at the same magnification. SAG, statoacoustic ganglion; am, anterior macula; ac, anterior crista; lc, lateral crista; pc, posterior crista.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
atoh1a AB standard conditions Prim-5 anterior macula ISH
AB + MO2-her9 standard conditions Prim-5 anterior macula ISH
GFP s356tTg standard conditions Long-pec posterior macula IFL
Day 4 anterior crista IFL
Day 4 lateral crista IFL
Day 4 posterior crista IFL
s356tTg + MO2-her9 standard conditions Long-pec posterior macula IFL
Day 4 anterior crista IFL
Day 4 lateral crista IFL
Day 4 posterior crista IFL
zc7Tg standard conditions Long-pec statoacoustic (VIII) ganglion IFL
zc7Tg + MO2-her9 standard conditions Long-pec statoacoustic (VIII) ganglion IFL
Antibody Labeling Details No data available
Phenotype Details No data available
Acknowledgments:
This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Development