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ZIRC
ZFIN ID: ZDB-FIG-110214-4
Radosevic et al., 2011 - Her9 represses neurogenic fate downstream of Tbx1 and retinoic acid signaling in the inner ear. Development (Cambridge, England)   138(3):397-408 Full text @ Development
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Antibody:
Fish:
Knockdown Reagent:
Anatomical Terms:
Stage: Prim-5

Fig. 4 her9 inhibits Cdkns and controls cell proliferation. (A-D) In situ hybridization of cdkn1bl (A,B) and cdkn1c (C,D) in wild-type (A,C) and her9-MO-injected (B,D) zebrafish embryos at 24 hpf. Arrow indicates posterolateral expansion of cdkn1bl expression. (E-H) Transverse sections of wild-type and Her9 morphant embryos stained by in situ hybridization for her9 (blue) and immunohistochemistry with anti-pH3 (green). (I) Proportion of pH3-positive cells counted inside and outside the her9-expression domain in wild-type and Her9-depleted embryos. Error bars indicate mean + s.e.m. *P=0.001. A-D and E-H are at the same magnification.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
cdkn1ba AB standard conditions Prim-5 otic vesicle ISH
AB + MO2-her9 standard conditions Prim-5 otic vesicle postero-lateral region ISH
cdkn1ca AB standard conditions Prim-5 otic vesicle ISH
AB + MO2-her9 standard conditions Prim-5 otic vesicle ISH
her9 AB standard conditions Prim-5 otic vesicle ISH
AB + MO2-her9 standard conditions Prim-5 otic vesicle ISH
Antibody Labeling Details
Antibody Assay Fish Conditions Stage Anatomy
Ab1-h3 IHC AB standard conditions Prim-5 otic vesicle
IHC AB + MO2-her9 standard conditions Prim-5 otic vesicle
Phenotype Details No data available
Acknowledgments:
ZFIN wishes to thank the journal Development (Cambridge, England) for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Development