Fig. 5

Tef regulates light-induced transcription.

(A) Transcript level analysis by qPCR after 9 hours of light exposure in morpholino microinjected tefα knock down embryos (grey bars) compared to untreated embryos (white bars). Asterisks indicate significant difference in expression level. (B) Experiment as in A for morpholino-mediated knock down of tefβ in light exposed embryos. Demonstrating the reduced effect of tefβ knock down on the levels of most light-induced transcripts when compared with tefα knock down. (C) Morpholino-mediated double knock down of tefα and tefβ in light exposed embryos matches the tefα knock down result. (D) Embryos microinjected with tefα mRNA and directly transferred to DD. tef over expression results in elevated transcript levels when compared to untreated embryos maintained in DD. Fold changes were normalized with DD transcript levels, thus the knock down, over expression, and wild type light exposed transcript levels are compared to the wild type expression level in DD, which is set at zero on the Y-axis within each bar. Differences between samples were corrected with β-actin mRNA levels. These data clearly demonstrate that Tef mediates the regulation of light-induced transcription.