Fig. 1

Screen for genes that display light-induced transcription.

(A) Scatter plot showing the fold change in transcript level between embryos (9 h.p.f.) that were exposed to light and siblings maintained in darkness. Each dot represents one transcript of 14,900 genes screened. The reliability of the data is indicated by a green to red colour scale, with only the red dots representing transcripts that have a trustworthy differential expression. The outer blue lines demarcate the 2.0 fold boundaries when related to the average (central blue line). (B) Pie chart representing the ratios of the different processes in which light-induced genes function. (C) Validation by qPCR (n = 7) of the 19 light-induced transcripts that were identified by the Affymetrix micro-array GeneChip (n = 3, fold change >2). Grey bars indicate micro-array fold change and orange bars indicate qPCR fold change. Error bars indicate the standard deviation in all experiments. The qPCR fold changes shown were normalised using DD transcript levels, and differences between samples were corrected with β-actin mRNA levels.