Fig. 4

Knockdown of smarcal1 reduces expression of hematopoietic genes. (A-H) WISH shows that expression of hemangioblast marker scl and hematopoietic stem cell marker gata2 were not affected by knockdown of smarcal1, whereas erythroid progenitor marker gata1 and erythroid marker β-E1 globin were significantly reduced. All embryos are in lateral view with anterior to the left. (I) Real-time RT-PCR results from 24 hpf embryos show that smarcal1 MO significantly reduced the expression of gata1 and β-E1 globin. (J-O) The expressions of myeloid progenitor marker pu.1, macrophage marker l-plastin and heterophil granulocyte marker mpo were not obviously changed by knockdown of smarcal1 at 18 hpf. (P) Real-time RT-PCR shows reduced expression of definitive erythroid marker runx1 in 24 hpf MO1 injected embryos. (Q-V) Knockdown of smarcal1 suppressed definitive hematopoiesis.. (Q-S) show the expression of pu.1, l-plastin and mpo at definitive myeloid cells and (T-V) show the markers were down-regulated by knockdown of smarcal1 at 30 hpf. The real time PCR results were obtained from at least three experiments. β-actin mRNA was measured as an internal control. Data are presented as means ± SE. low asteriskP < 0.05.