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Fig. 2

ID

ZDB-FIG-100309-2

Publication

Huang et al., 2010 - Deficiency of smarcal1 causes cell cycle arrest and developmental abnormalities in zebrafish

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Fig. 2

Design and efficiency of smarcal1 MOs. (A) Design of smarcal1 MOs and RT-PCR primers for detecting the splice-blocked smarcal1 mRNA. P1 and P2 indicate the primers used in (D). (B) Embryos with injection of smarcal1 5′ UTR-5′cds-GFP mRNA (50 pg per embryo) displayed green fluorescence, whereas co-injection of smarcal1 MO1 (8 ng per embryo) with 5′ UTR-5′cds-GFP mRNA (50 pg per embryo) blocked fluorescence signal. (C) Knockdown efficiency of smarca11 MO1 analyzed by Western blot with V5 antibody. V5-tagged full-length zebrafish smarcal1 injected embryos (200 pg per embryo) showed the induction of 85 KD protein, whereas co-injection with 8 ng of MO1 inhibited the protein translation. A 105 KD non-specific band and β-actin serve as loading control. (D) RT-PCR analysis of splice-blocking efficiency of MO2. A 640 bp product was amplified from WT embryos and MO2 deleted about 70 nucleotides of smarcal1 mRNA. In each group, 20 embryos were pooled for western blot and RT-PCR experiments.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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Reprinted from Developmental Biology, 339(1), Huang, C., Gu, S., Yu, P., Yu, F., Feng, C., Gao, N., and Du, J., Deficiency of smarcal1 causes cell cycle arrest and developmental abnormalities in zebrafish, 89-100, Copyright (2010) with permission from Elsevier. Full text @ Dev. Biol.