ZFIN ID: ZDB-FIG-100223-42
Raeker et al., 2010 - Targeted deletion of the zebrafish obscurin A RhoGEF domain affects heart, skeletal muscle and brain development. Developmental Biology   337(2):432-443 Full text @ Dev. Biol.
ADDITIONAL FIGURES
PHENOTYPE:
Fish:
Knockdown Reagents:
Observed In:
Stage: Protruding-mouth

Fig. 4 Skeletal muscle phenotype in obscurin A RhoGEF morphant embryos at 72 hpf. Ultrastructural analysis was performed at 72 hpf on control, obscurin A (ObscMO) and obscurin A RhoGEF (ObscRhoMO) morphant embryos. In control embryos, the myofibrils are aligned in register across the sarcoplasm (A) with well defined M bands and finely “stitched” Z disks (D). In the obscurin morphant embryos (B) there is marked disturbance of myofibril organization with areas of misalignment of the M bands of adjacent myofibrils (E: ^). By comparison, the obscurin A RhoGEF morphants display myofibril organization (C) similar to control embryos but reduced myofibrillar content with more sarcoplasm devoid of myofibrils and more rounded nuclei (C, G: ^). There were also mild sarcomeric abnormalities including, infrequently, an indistinct appearance of the Z disk and absence of the M band (F: ∗) which could occur even within myofibrils demonstrating mature Z disks and M bands (F: ^). Note that in skeletal myocytes stained with DAPI, the nuclei in the obscurin A RhoGEF morphants (RhoMO) are closer together and more rounded (G) than in controls (H) consistent with reduced myofibril volume. Scale bars 2 μm (A–C), 0.5 μm (D–F) and 10 μm (G, H).

Gene Expression Details No data available
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
WT + MO2-obscnb standard conditions Protruding-mouth skeletal muscle cell myofibril disorganized, abnormal
WT + MO3-obscnb + MO4-obscnb standard conditions Protruding-mouth skeletal muscle cell M band decreased amount, abnormal
Protruding-mouth skeletal muscle cell myofibril decreased amount, abnormal
Protruding-mouth skeletal muscle cell nucleus shape, abnormal
Protruding-mouth skeletal muscle cell sarcomere structure, abnormal
Protruding-mouth skeletal muscle cell Z disc structure, abnormal
Acknowledgments:
ZFIN wishes to thank the journal Developmental Biology for permission to reproduce figures from this article. Please note that this material may be protected by copyright.

Reprinted from Developmental Biology, 337(2), Raeker, M.O., Bieniek, A.N., Ryan, A.S., Tsai, H.J., Zahn, K.M., and Russell, M.W., Targeted deletion of the zebrafish obscurin A RhoGEF domain affects heart, skeletal muscle and brain development, 432-443, Copyright (2010) with permission from Elsevier. Full text @ Dev. Biol.