FIGURE

Fig. 5

ID

ZDB-FIG-100223-43

Publication

Raeker et al., 2010 - Targeted deletion of the zebrafish obscurin A RhoGEF domain affects heart, skeletal muscle and brain development

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Fig. 5

Absence of consistent M bands in obscurin A RhoGEF skeletal muscle at 72 hpf. Control embryos (A) consistently demonstrate well-formed M bands (>) in skeletal myofibrils by 72 hpf. Obscurin A morphant embryos (B), although they display marked abnormalities in myofibrillar architecture, also consistently demonstrate mature M bands (>). In contrast, skeletal myofibrils in obscurin A RhoGEF morphant embryos (C) demonstrated occasional absence of M bands (∗) in otherwise mature-appearing myofibrils. Note that the extrajunctional SR in the ObscMO embryo is markedly disorganized (B: arrow), but is much more normal appearing in the ObscRhoMO embryos (C: arrow).

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	WT + MO2-obscnb WT + MO3-obscnb + MO4-obscnb
Knockdown Reagents:	MO2-obscnb MO3-obscnb MO4-obscnb
Observed In:	skeletal muscle cell M band skeletal muscle cell sarcoplasmic reticulum
Stage:	Protruding-mouth

Phenotype Detail

Acknowledgments

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Reprinted from Developmental Biology, 337(2), Raeker, M.O., Bieniek, A.N., Ryan, A.S., Tsai, H.J., Zahn, K.M., and Russell, M.W., Targeted deletion of the zebrafish obscurin A RhoGEF domain affects heart, skeletal muscle and brain development, 432-443, Copyright (2010) with permission from Elsevier. Full text @ Dev. Biol.