ZFIN ID: ZDB-FIG-100223-43
Raeker et al., 2010 - Targeted deletion of the zebrafish obscurin A RhoGEF domain affects heart, skeletal muscle and brain development. Developmental Biology   337(2):432-443 Full text @ Dev. Biol.
ADDITIONAL FIGURES
PHENOTYPE:
Fish:
Knockdown Reagents:
Observed In:
Stage: Protruding-mouth

Fig. 5 Absence of consistent M bands in obscurin A RhoGEF skeletal muscle at 72 hpf. Control embryos (A) consistently demonstrate well-formed M bands (>) in skeletal myofibrils by 72 hpf. Obscurin A morphant embryos (B), although they display marked abnormalities in myofibrillar architecture, also consistently demonstrate mature M bands (>). In contrast, skeletal myofibrils in obscurin A RhoGEF morphant embryos (C) demonstrated occasional absence of M bands (∗) in otherwise mature-appearing myofibrils. Note that the extrajunctional SR in the ObscMO embryo is markedly disorganized (B: arrow), but is much more normal appearing in the ObscRhoMO embryos (C: arrow).

Gene Expression Details No data available
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
WT + MO2-obscnb standard conditions Protruding-mouth skeletal muscle cell sarcoplasmic reticulum disorganized, abnormal
WT + MO3-obscnb + MO4-obscnb standard conditions Protruding-mouth skeletal muscle cell M band decreased amount, abnormal
Acknowledgments:
ZFIN wishes to thank the journal Developmental Biology for permission to reproduce figures from this article. Please note that this material may be protected by copyright.

Reprinted from Developmental Biology, 337(2), Raeker, M.O., Bieniek, A.N., Ryan, A.S., Tsai, H.J., Zahn, K.M., and Russell, M.W., Targeted deletion of the zebrafish obscurin A RhoGEF domain affects heart, skeletal muscle and brain development, 432-443, Copyright (2010) with permission from Elsevier. Full text @ Dev. Biol.