ZFIN ID: ZDB-FIG-100223-45
Raeker et al., 2010 - Targeted deletion of the zebrafish obscurin A RhoGEF domain affects heart, skeletal muscle and brain development. Developmental Biology   337(2):432-443 Full text @ Dev. Biol.
ADDITIONAL FIGURES
PHENOTYPE:
Fish:
Knockdown Reagents:
Observed In:
Stage: Protruding-mouth

Fig. 7 Ultrastructural analysis of cardiac myocytes at 72 hpf. (A) Cardiac myocytes in control embryos have formed intercalated disks (^) and filled the sarcoplasm with mature myofibrils. (B) Higher magnification of an intercalated disk (ID) from a control embryo demonstrates adherens (>) and gap (^) junctions. (C, D) In contrast, cardiac myocytes from mildly affected obscurin A RhoGEF morphant embryos have reduced myofibrillar content [compare the nuclear (N) area to the myofibrillar area within each myocyte] and very poorly defined regions of cell–cell (^) contact with no identifiable IDs. Examples of recent nuclear division (N+) were more frequent in the obscurin RhoGEF morphant embryos. (E, F) In more severely affected embryos, cardiac myocytes appear markedly abnormal with loosely arranged contractile filaments (F: ∗). (G, H) The mature cardiac myofibrils that did form in the obscurin A RhoGEF morphant embryos were not significantly different than control with a normal appearing lattice of thick filaments on cross-section (G) and laterally aligned Z disks and M bands on longitudinal section (H). Scale bars are 4 μm (A–C), 2 μm (D, E), 1 μm (F) and 0.5 μm (G, H).

Gene Expression Details No data available
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
WT + MO3-obscnb + MO4-obscnb standard conditions Protruding-mouth cardiac muscle cell structure, abnormal
Protruding-mouth cardiac muscle cell intercalated disc absent, abnormal
Protruding-mouth cardiac muscle cell myofibril decreased amount, abnormal
Acknowledgments:
ZFIN wishes to thank the journal Developmental Biology for permission to reproduce figures from this article. Please note that this material may be protected by copyright.

Reprinted from Developmental Biology, 337(2), Raeker, M.O., Bieniek, A.N., Ryan, A.S., Tsai, H.J., Zahn, K.M., and Russell, M.W., Targeted deletion of the zebrafish obscurin A RhoGEF domain affects heart, skeletal muscle and brain development, 432-443, Copyright (2010) with permission from Elsevier. Full text @ Dev. Biol.