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ZFIN ID: ZDB-FIG-100223-44
Raeker et al., 2010 - Targeted deletion of the zebrafish obscurin A RhoGEF domain affects heart, skeletal muscle and brain development. Developmental Biology   337(2):432-443 Full text @ Dev. Biol.
Knockdown Reagents:
Observed In:
Stage Range: Long-pec to Protruding-mouth

Fig. 6 Obscurin A RhoGEF signaling is required for normal cardiac development. Cmlc2-EGFP transgenic zebrafish embryos injected with obscurin A RhoGEF morpholinos (B–F) were compared to control-injected transgenic embryos (A) at 72 hpf. Morphant embryos demonstrated a spectrum of cardiac abnormalities ranging from reduced cardiac function with cardiac dilatation and mild pericardial edema (B–D) to mild (E) or severe (F) ventricular hypoplasia and pericardial edema (<). (G–J) Cardiac functional assessments were performed in morphant (black bars) and control embryos (white bars) at 48 and 72 hpf. In the more mildly affected embryos with mild ventricular hypoplasia as determined by comparable end diastolic volume measurements relative to controls (I), there were significant reductions in heart rate (G), ejection fraction (H) and stroke volume (J) at 48 and 72 hpf compared to controls (∗t-test; p < 0.05).

Gene Expression DetailsNo data available
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
twu34Tg + MO3-obscnb + MO4-obscnb standard conditions Long-pec - Protruding-mouth heart decreased functionality, abnormal
Long-pec - Protruding-mouth heart contraction decreased rate, abnormal
Protruding-mouth cardiac ventricle decreased size, abnormal
Protruding-mouth heart dilated, abnormal
Protruding-mouth pericardium edematous, abnormal
ZFIN wishes to thank the journal Developmental Biology for permission to reproduce figures from this article. Please note that this material may be protected by copyright.

Reprinted from Developmental Biology, 337(2), Raeker, M.O., Bieniek, A.N., Ryan, A.S., Tsai, H.J., Zahn, K.M., and Russell, M.W., Targeted deletion of the zebrafish obscurin A RhoGEF domain affects heart, skeletal muscle and brain development, 432-443, Copyright (2010) with permission from Elsevier. Full text @ Dev. Biol.