ZFIN ID: ZDB-FIG-100223-41
Raeker et al., 2010 - Targeted deletion of the zebrafish obscurin A RhoGEF domain affects heart, skeletal muscle and brain development. Developmental Biology   337(2):432-443 Full text @ Dev. Biol.
Knockdown Reagents:
Observed In:
Stage: Protruding-mouth

Fig. 3 Architecture of the myotendinous junction (MTJ). Confocal analysis using α-actinin (a Z disk component) immunolabeling (A–C) to assess myofibril morphology and ultrastructural analysis (D–F) to define the architecture of the MTJ was performed in control (A, D), obscurin A morphant (B, E) and obscurin A RhoGEF morphant (C, F) embryos at 72 hpf. In control embryos, the skeletal myocytes are aligned in parallel and the myotendinous junctions are well defined (A, D: >). The skeletal myocytes in obscurin A (ObscMO) morphant embryos vary in length and in their relationship to each other (∗) with elongated myocytes extending past very rudimentary somite boundaries (B, E: >). When compared to the ObscMO embryos, the obscurin A RhoGEF (ObscRhoMO) morphant embryos demonstrate a more consistent relationship between adjacent myocyte (more uniform parallel arrangement) and normal positioning of a complete or nearly complete somite boundary (C,F: >). However, the boundary is not sharply defined as in control embryos and often appears electron dense and irregular, not unlike the rudimentary boundaries/MTJs noted in ObscMO embryos. Scale bars are 20 μm (A–C) and 2 μm (D–F).

Gene Expression Details No data available
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
WT + MO2-obscnb standard conditions Protruding-mouth skeletal muscle cell elongated, abnormal
Protruding-mouth vertical myoseptum disorganized, abnormal
WT + MO3-obscnb + MO4-obscnb standard conditions Protruding-mouth vertical myoseptum disorganized, abnormal
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Reprinted from Developmental Biology, 337(2), Raeker, M.O., Bieniek, A.N., Ryan, A.S., Tsai, H.J., Zahn, K.M., and Russell, M.W., Targeted deletion of the zebrafish obscurin A RhoGEF domain affects heart, skeletal muscle and brain development, 432-443, Copyright (2010) with permission from Elsevier. Full text @ Dev. Biol.