ZFIN ID: ZDB-FIG-100223-41
Raeker et al., 2010 - Targeted deletion of the zebrafish obscurin A RhoGEF domain affects heart, skeletal muscle and brain development. Developmental Biology   337(2):432-443 Full text @ Dev. Biol.
ADDITIONAL FIGURES
PHENOTYPE:
Fish:
Knockdown Reagents:
Observed In:
Stage: Protruding-mouth

Fig. 3 Architecture of the myotendinous junction (MTJ). Confocal analysis using α-actinin (a Z disk component) immunolabeling (A–C) to assess myofibril morphology and ultrastructural analysis (D–F) to define the architecture of the MTJ was performed in control (A, D), obscurin A morphant (B, E) and obscurin A RhoGEF morphant (C, F) embryos at 72 hpf. In control embryos, the skeletal myocytes are aligned in parallel and the myotendinous junctions are well defined (A, D: >). The skeletal myocytes in obscurin A (ObscMO) morphant embryos vary in length and in their relationship to each other (∗) with elongated myocytes extending past very rudimentary somite boundaries (B, E: >). When compared to the ObscMO embryos, the obscurin A RhoGEF (ObscRhoMO) morphant embryos demonstrate a more consistent relationship between adjacent myocyte (more uniform parallel arrangement) and normal positioning of a complete or nearly complete somite boundary (C,F: >). However, the boundary is not sharply defined as in control embryos and often appears electron dense and irregular, not unlike the rudimentary boundaries/MTJs noted in ObscMO embryos. Scale bars are 20 μm (A–C) and 2 μm (D–F).

Gene Expression Details No data available
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
WT + MO2-obscnb standard conditions Protruding-mouth skeletal muscle cell elongated, abnormal
Protruding-mouth vertical myoseptum disorganized, abnormal
WT + MO3-obscnb + MO4-obscnb standard conditions Protruding-mouth vertical myoseptum disorganized, abnormal
Acknowledgments:
ZFIN wishes to thank the journal Developmental Biology for permission to reproduce figures from this article. Please note that this material may be protected by copyright.

Reprinted from Developmental Biology, 337(2), Raeker, M.O., Bieniek, A.N., Ryan, A.S., Tsai, H.J., Zahn, K.M., and Russell, M.W., Targeted deletion of the zebrafish obscurin A RhoGEF domain affects heart, skeletal muscle and brain development, 432-443, Copyright (2010) with permission from Elsevier. Full text @ Dev. Biol.