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ZFIN ID: ZDB-FIG-090915-28
Maves et al., 2009 - Pbx acts with Hand2 in early myocardial differentiation. Developmental Biology   333(2):409-418 Full text @ Dev. Biol.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Knockdown Reagents:
Anatomical Terms:
Stage Range: 10-13 somites to 20-25 somites

Fig. 2 Validation of microarray-identified Pbx-dependent heart genes. (A–R) RNA in situ expression of genes from Table 1 in (A, C, E, G, I, K, M, O, Q) wild-type control or (B, D, F, H, J, L, N, P, R) pbx2-MO; pbx4-MO embryos. (A–D) are at 10 somite stage (10s). (E–N,Q–R) are at 18s. (O–P) are at 20s to achieve more robust expression of lft1 in controls. krox-20, labeled in hindbrain rhombomeres r3 and r5 in (A), is included in all in situs to control for loss of Pbx. The wild-type heart primordium expression domains of tnnt2, ttna, cmlc1, vmhc, myl7, lft1 and hand2 are just anterior-lateral to r3 krox-20 expression (arrows in A, O, Q). n  ≥ 10 for each marker in control or pbx2-MO; pbx4-MO. Embryos are shown in dorsal view, anterior towards the left.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
aldh1a2 AB control 14-19 somites lateral plate mesoderm ISH
AB + MO1-pbx2 + MO2-pbx2 + MO2-pbx4 + MO3-pbx4 standard conditions 14-19 somites lateral plate mesoderm ISH
cmlc1 AB control 14-19 somites heart primordium ISH
14-19 somites somite ISH
AB + MO1-pbx2 + MO2-pbx2 + MO2-pbx4 + MO3-pbx4 standard conditions 14-19 somites heart primordium ISH
14-19 somites somite ISH
hand2 AB control 20-25 somites heart primordium ISH
20-25 somites ventral mesoderm ISH
AB + MO1-pbx2 + MO2-pbx2 + MO2-pbx4 + MO3-pbx4 standard conditions 20-25 somites heart primordium ISH
20-25 somites ventral mesoderm ISH
lft1 AB control 20-25 somites forebrain ISH
20-25 somites heart rudiment ISH
20-25 somites notochord ISH
AB + MO1-pbx2 + MO2-pbx2 + MO2-pbx4 + MO3-pbx4 standard conditions 20-25 somites forebrain ISH
20-25 somites heart rudiment ISH
20-25 somites notochord ISH
myh7 AB control 14-19 somites heart primordium ISH
14-19 somites somite ISH
AB + MO1-pbx2 + MO2-pbx2 + MO2-pbx4 + MO3-pbx4 standard conditions 14-19 somites heart primordium ISH
14-19 somites somite ISH
myl7 AB control 14-19 somites heart primordium ISH
AB + MO1-pbx2 + MO2-pbx2 + MO2-pbx4 + MO3-pbx4 standard conditions 14-19 somites heart primordium ISH
tnnt2a AB control 10-13 somites heart primordium ISH
AB + MO1-pbx2 + MO2-pbx2 + MO2-pbx4 + MO3-pbx4 standard conditions 10-13 somites heart primordium ISH
ttn.2 AB control 10-13 somites heart primordium ISH
10-13 somites somite ISH
AB + MO1-pbx2 + MO2-pbx2 + MO2-pbx4 + MO3-pbx4 standard conditions 10-13 somites heart primordium ISH
10-13 somites somite ISH
Antibody Labeling Details No data available
Phenotype Details No data available
Acknowledgments:
ZFIN wishes to thank the journal Developmental Biology for permission to reproduce figures from this article. Please note that this material may be protected by copyright.

Reprinted from Developmental Biology, 333(2), Maves, L., Tyler, A., Moens, C.B., and Tapscott, S.J., Pbx acts with Hand2 in early myocardial differentiation, 409-418, Copyright (2009) with permission from Elsevier. Full text @ Dev. Biol.