FIGURE

Fig. 4

ID

ZDB-FIG-090915-30

Publication

Maves et al., 2009 - Pbx acts with Hand2 in early myocardial differentiation

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Fig. 4

Pbx-deficient and hand2-MO embryos exhibit similar cardiac differentiation and morphogenesis defects. (A–J) RNA in situ expression of (A,D,G) myl7, (B,E,H) vmhc, (C,F,I) amhc, and (J) nppa at (A–C) 21 somites, (D–F) 25 h post fertilization (hpf), (G–I) 42 hpf, and (J) 28 hpf. In control embryos shown, myl7 labels all myocardial precursors, vmhc labels ventricular precursors, and amhc labels atrial precursors (Berdougo et al., 2003). Ventricle (v) and atrium (a) are labeled in (G). n ≥ 20 for each marker in control, pbx2-MO; pbx4-MO, or hand2-MO. (A–F) Embryos are shown in dorsal view, anterior towards the left. (G–I) Embryos are shown in frontal views, dorsal towards the right.

Expression Data

Genes:	myh6 myh7 myl7 nppa
Fish:	AB AB + MO2-hand2 AB + MO1-pbx2 + MO2-pbx2 + MO2-pbx4 + MO3-pbx4
Knockdown Reagents:	MO1-pbx2 MO2-hand2 MO2-pbx2 MO2-pbx4 MO3-pbx4
Anatomical Terms:	atrium cardiac muscle cell cardiac ventricle heart heart rudiment heart tube myocardial precursor presumptive atrium heart tube presumptive cardiac ventricle heart tube
Stage Range:	20-25 somites to High-pec

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	AB + MO2-hand2 AB + MO1-pbx2 + MO2-pbx2 + MO2-pbx4 + MO3-pbx4
Knockdown Reagents:	MO1-pbx2 MO2-hand2 MO2-pbx2 MO2-pbx4 MO3-pbx4
Observed In:	atrium cardiac muscle cell differentiation cardiac ventricle cell migration to the midline involved in heart development embryonic heart tube elongation heart development heart looping heart morphogenesis heart primordium heart primordium myocardial precursor heart tube
Stage Range:	20-25 somites to High-pec

Phenotype Detail

Acknowledgments

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Reprinted from Developmental Biology, 333(2), Maves, L., Tyler, A., Moens, C.B., and Tapscott, S.J., Pbx acts with Hand2 in early myocardial differentiation, 409-418, Copyright (2009) with permission from Elsevier. Full text @ Dev. Biol.