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ZFIN ID: ZDB-FIG-090519-9
Kitaguchi et al., 2009 - Transcriptional regulation of a myeloid-lineage specific gene lysozyme C during zebrafish myelopoiesis. Mechanisms of Development   126(5-6):314-323 Full text @ Mech. Dev.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Knockdown Reagents:
Anatomical Terms:
Stage: Prim-5

Fig. 3 Differential requirement of myeloid-expressed transcription factor on the expression of c/ebp1, pu.1 and runx1. Whole-mount in situ hybridization with probes shown at bottom right of each panel. Injected morpholinos are indicated at top right. All embryos are around 24 hpf and lateral view; anterior is left. (A–D) lyz expression was inhibited in pu.1 MO-, runx1 MO- and c/ebp1 MO-injected embryos. (E–G) The expression of c/ebp1 in myeloid cells was suppressed in pu.1 morphants, but not in runx1 morphants. (H–J) The runx1 expression in myeloid cells (red arrowheads; H and J), but not in the ICM and Rohon-beard cells, was inhibited in pu.1 morphants, while injection of c/ebp1 MO did not affect runx1 expression. (K–M) pu.1 expression in myeloid cells was comparably detected in uninjected-, runx1 MO-injected and c/ebp1 MO-injected embryos.

Gene Expression Details
Gene Antibody Fish Conditions Stage Qualifier Anatomy Assay
cebp1 WT standard conditions Prim-5 myeloid cell ISH
WT + MO1-spi1b standard conditions Prim-5 Not Detected myeloid cell ISH
WT + MO4-runx1 standard conditions Prim-5 myeloid cell ISH
lyz WT standard conditions Prim-5 myeloid cell ISH
WT + MO1-spi1b standard conditions Prim-5 Not Detected myeloid cell ISH
WT + MO3-cebp1 standard conditions Prim-5 Not Detected myeloid cell ISH
WT + MO4-runx1 standard conditions Prim-5 Not Detected myeloid cell ISH
runx1 WT standard conditions Prim-5 intermediate cell mass of mesoderm ISH
Prim-5 myeloid cell ISH
Prim-5 Rohon-Beard neuron ISH
WT + MO1-spi1b standard conditions Prim-5 intermediate cell mass of mesoderm ISH
Prim-5 Not Detected myeloid cell ISH
Prim-5 Rohon-Beard neuron ISH
WT + MO3-cebp1 standard conditions Prim-5 intermediate cell mass of mesoderm ISH
Prim-5 myeloid cell ISH
Prim-5 Rohon-Beard neuron ISH
spi1b WT standard conditions Prim-5 myeloid cell ISH
WT + MO3-cebp1 standard conditions Prim-5 myeloid cell ISH
WT + MO4-runx1 standard conditions Prim-5 myeloid cell ISH
Antibody Labeling Details No data available
Phenotype Details No data available
Acknowledgments:
ZFIN wishes to thank the journal Mechanisms of Development for permission to reproduce figures from this article. Please note that this material may be protected by copyright.

Reprinted from Mechanisms of Development, 126(5-6), Kitaguchi, T., Kawakami, K., and Kawahara, A., Transcriptional regulation of a myeloid-lineage specific gene lysozyme C during zebrafish myelopoiesis, 314-323, Copyright (2009) with permission from Elsevier. Full text @ Mech. Dev.