ZFIN ID: ZDB-FIG-090519-10
c/ebp1, pu.1 and runx1 coordinately regulate lyz expression. All embryos are around 24 hpf and lateral view; anterior is left. (A) Injection of mRNA mixture (runx1 [100 pg] + pu.1 [10 pg] or runx1 [100 pg] + c/ebp1 [100 pg]) did not affect lyz expression compared to uninjected embryos. lyz expression in the ICM (orange arrowhead) was weakly induced in the embryos injected with mRNA mixture (c/ebp1 [100 pg] + pu.1 [10 pg]). Injection of mRNA mixture (runx1 [100 pg] + c/ebp1 [100 pg] + pu.1 [10 pg]) strongly induced lyz expansion in the ICM (red arrowhead). (B) The expression of erythroid markers gata1 and band3 was comparable in both uninjected- and triple mRNA-injected embryos.
Antibody Labeling Details No data available
Phenotype Details No data available
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Reprinted from Mechanisms of Development, 126(5-6), Kitaguchi, T., Kawakami, K., and Kawahara, A., Transcriptional regulation of a myeloid-lineage specific gene lysozyme C during zebrafish myelopoiesis, 314-323, Copyright
(2009) with permission from Elsevier.
Full text @ Mech. Dev.