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ZFIN ID: ZDB-FIG-090519-10
Kitaguchi et al., 2009 - Transcriptional regulation of a myeloid-lineage specific gene lysozyme C during zebrafish myelopoiesis. Mechanisms of Development   126(5-6):314-323 Full text @ Mech. Dev.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Anatomical Terms:
Stage: Prim-5

Fig. 4 c/ebp1, pu.1 and runx1 coordinately regulate lyz expression. All embryos are around 24 hpf and lateral view; anterior is left. (A) Injection of mRNA mixture (runx1 [100 pg] + pu.1 [10 pg] or runx1 [100 pg] + c/ebp1 [100 pg]) did not affect lyz expression compared to uninjected embryos. lyz expression in the ICM (orange arrowhead) was weakly induced in the embryos injected with mRNA mixture (c/ebp1 [100 pg] + pu.1 [10 pg]). Injection of mRNA mixture (runx1 [100 pg] + c/ebp1 [100 pg] + pu.1 [10 pg]) strongly induced lyz expansion in the ICM (red arrowhead). (B) The expression of erythroid markers gata1 and band3 was comparable in both uninjected- and triple mRNA-injected embryos.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
gata1a WT standard conditions Prim-5 intermediate cell mass of mesoderm ISH
lyz WT standard conditions Prim-5 myeloid cell ISH
slc4a1a WT standard conditions Prim-5 intermediate cell mass of mesoderm ISH
Antibody Labeling Details No data available
Phenotype Details No data available
Acknowledgments:
ZFIN wishes to thank the journal Mechanisms of Development for permission to reproduce figures from this article. Please note that this material may be protected by copyright.

Reprinted from Mechanisms of Development, 126(5-6), Kitaguchi, T., Kawakami, K., and Kawahara, A., Transcriptional regulation of a myeloid-lineage specific gene lysozyme C during zebrafish myelopoiesis, 314-323, Copyright (2009) with permission from Elsevier. Full text @ Mech. Dev.