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ZFIN ID: ZDB-FIG-090519-12
Kitaguchi et al., 2009 - Transcriptional regulation of a myeloid-lineage specific gene lysozyme C during zebrafish myelopoiesis. Mechanisms of Development   126(5-6):314-323 Full text @ Mech. Dev.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Knockdown Reagents:
Anatomical Terms:
Stage: Prim-5

Fig. 6 Suppression of erythroid-lineage factors biklf and gata1 strongly induces ectopic lyz expression in the ICM. (A–P) All embryos are around 24 hpf and lateral view; anterior is left. Morpholinos (biklf MO; 10 ng) were injected into one-cell stage embryos from wild-type or vlad tepes (vlt)/gata1 mutant, and the expression of myeloid markers was examined by whole-mount in situ hybridization; marker is shown at bottom right. Genome DNA was prepared from the stained embryos after taking pictures, and genotyping of vlt was performed. The myeloid markers, pu.1, c/ebp1, runx1 and lyz are expressed in myeloid cells over the yolk (orange arrowheads) (A, E and M). The expression of pu.1, c/ebp1, runx1 and lyz was weakly induced in the ICM (red arrowheads) in vlt embryos or biklf MO-injected embryos. When biklf MO was injected into vlt embryos, the markers (pu.1, c/ebp1, runx1 and lyz) were strongly induced in the ICM (red arrowheads). (Q and R) EGFP expression is detected in myeloid cells (orange arrowheads) in uninjected embryo from Tg(lyz:EGFP)ko02. EGFP expression at 23 hpf stage was significantly induced in the ICM (white arrowheads), when morpholinos (gata1 MO; 10 ng + biklf MO; 10 ng) were injected into one-cell stage embryos of Tg(lyz:EGFP)ko02. (S and T) mRFP expression in the ALPM (orange arrowheads) at 23 hpf stage was slightly increased, when morpholinos (pu.1 MO; 10 ng + c/ebp1 MO; 10 ng) were injected into one-cell stage embryos of Tg(gata1:mRFP)ko05.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
cebp1 WT standard conditions Prim-5 myeloid cell ISH
WT + MO1-klf17 standard conditions Prim-5 intermediate cell mass of mesoderm ISH
gata1am651/m651 standard conditions Prim-5 intermediate cell mass of mesoderm ISH
gata1am651/m651 + MO1-klf17 standard conditions Prim-5 intermediate cell mass of mesoderm ISH
EGFP ko02Tg standard conditions Prim-5 myeloid cell IFL
ko02Tg + MO1-gata1a + MO1-klf17 standard conditions Prim-5 intermediate cell mass of mesoderm IFL
lyz WT standard conditions Prim-5 myeloid cell ISH
WT + MO1-klf17 standard conditions Prim-5 intermediate cell mass of mesoderm ISH
gata1am651/m651 standard conditions Prim-5 intermediate cell mass of mesoderm ISH
gata1am651/m651 + MO1-klf17 standard conditions Prim-5 intermediate cell mass of mesoderm ISH
mRFP ko05Tg standard conditions Prim-5 anterior lateral plate mesoderm IFL
Prim-5 intermediate cell mass of mesoderm IFL
ko05Tg + MO1-spi1b + MO3-cebp1 standard conditions Prim-5 anterior lateral plate mesoderm IFL
Prim-5 intermediate cell mass of mesoderm IFL
runx1 WT standard conditions Prim-5 myeloid cell ISH
WT + MO1-klf17 standard conditions Prim-5 intermediate cell mass of mesoderm ISH
gata1am651/m651 standard conditions Prim-5 intermediate cell mass of mesoderm ISH
gata1am651/m651 + MO1-klf17 standard conditions Prim-5 intermediate cell mass of mesoderm ISH
spi1b WT standard conditions Prim-5 myeloid cell ISH
WT + MO1-klf17 standard conditions Prim-5 intermediate cell mass of mesoderm ISH
gata1am651/m651 standard conditions Prim-5 intermediate cell mass of mesoderm ISH
gata1am651/m651 + MO1-klf17 standard conditions Prim-5 intermediate cell mass of mesoderm ISH
Antibody Labeling Details No data available
Phenotype Details No data available
Acknowledgments:
ZFIN wishes to thank the journal Mechanisms of Development for permission to reproduce figures from this article. Please note that this material may be protected by copyright.

Reprinted from Mechanisms of Development, 126(5-6), Kitaguchi, T., Kawakami, K., and Kawahara, A., Transcriptional regulation of a myeloid-lineage specific gene lysozyme C during zebrafish myelopoiesis, 314-323, Copyright (2009) with permission from Elsevier. Full text @ Mech. Dev.