ZFIN ID: ZDB-FIG-090519-11
Establishment of the lyz promoter-EGFP transgenic zebrafish, Tg(lyz:EGFP)ko02. All pictures show lateral view; anterior is left. (A, B and F–I) Fluorescent images. A stable transgenic fish line Tg(lyz:EGFP)ko02 expressing EGFP driven by the 2.4 kb lyz promoter. (A and B) EGFP-positive cells in Tg(lyz:EGFP)ko02 at 26 hpf (A) or 3 dpf (B). (C–E) Two-color in situ hybridization with probes of EGFP and lyz: EGFP expression stained by FITC (green) completely overlapped endogenous lyz expression stained by FastRed (red). (F–I) Effect of morpholinos (runx1 MO, pu.1 MO and c/ebp1 MO) and mRNAs (runx1 + pu.1 + c/ebp1) on EGFP expression in Tg(lyz:EGFP)ko02. Injected mRNAs or morpholinos are indicated at top right. The EGFP expression in Tg(lyz:EGFP)ko02 was strongly suppressed in runx1 MO (0.5 ng)-injected, pu.1 MO (10 ng)-injected, or c/ebp1 MO (10 ng)-injected embryo compared to uninjected embryos (A). In contrast, the number of EGFP-positive cells increased in the embryos injected with mRNA (runx1 [100 pg] + c/ebp1 [100 pg] + pu.1 [10 pg]) in Tg(lyz:EGFP)ko02.
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Reprinted from Mechanisms of Development, 126(5-6), Kitaguchi, T., Kawakami, K., and Kawahara, A., Transcriptional regulation of a myeloid-lineage specific gene lysozyme C during zebrafish myelopoiesis, 314-323, Copyright
(2009) with permission from Elsevier.
Full text @ Mech. Dev.