header logo image header logo text
Downloads Login
Research
General Information
ZIRC
ZFIN ID: ZDB-FIG-090519-11
Kitaguchi et al., 2009 - Transcriptional regulation of a myeloid-lineage specific gene lysozyme C during zebrafish myelopoiesis. Mechanisms of Development   126(5-6):314-323 Full text @ Mech. Dev.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Gene:
Fish:
Knockdown Reagents:
Anatomical Term:
Stage Range: Prim-5 to Protruding-mouth

Fig. 5 Establishment of the lyz promoter-EGFP transgenic zebrafish, Tg(lyz:EGFP)ko02. All pictures show lateral view; anterior is left. (A, B and F–I) Fluorescent images. A stable transgenic fish line Tg(lyz:EGFP)ko02 expressing EGFP driven by the 2.4 kb lyz promoter. (A and B) EGFP-positive cells in Tg(lyz:EGFP)ko02 at 26 hpf (A) or 3 dpf (B). (C–E) Two-color in situ hybridization with probes of EGFP and lyz: EGFP expression stained by FITC (green) completely overlapped endogenous lyz expression stained by FastRed (red). (F–I) Effect of morpholinos (runx1 MO, pu.1 MO and c/ebp1 MO) and mRNAs (runx1 + pu.1 + c/ebp1) on EGFP expression in Tg(lyz:EGFP)ko02. Injected mRNAs or morpholinos are indicated at top right. The EGFP expression in Tg(lyz:EGFP)ko02 was strongly suppressed in runx1 MO (0.5 ng)-injected, pu.1 MO (10 ng)-injected, or c/ebp1 MO (10 ng)-injected embryo compared to uninjected embryos (A). In contrast, the number of EGFP-positive cells increased in the embryos injected with mRNA (runx1 [100 pg] + c/ebp1 [100 pg] + pu.1 [10 pg]) in Tg(lyz:EGFP)ko02.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
EGFP ko02Tg standard conditions Prim-5 myeloid cell IFL
Protruding-mouth myeloid cell IFL
ko02Tg + MO1-spi1b standard conditions Prim-5 myeloid cell IFL
ko02Tg + MO3-cebp1 standard conditions Prim-5 myeloid cell IFL
ko02Tg + MO4-runx1 standard conditions Prim-5 myeloid cell IFL
Antibody Labeling Details No data available
Phenotype Details No data available
Acknowledgments:
ZFIN wishes to thank the journal Mechanisms of Development for permission to reproduce figures from this article. Please note that this material may be protected by copyright.

Reprinted from Mechanisms of Development, 126(5-6), Kitaguchi, T., Kawakami, K., and Kawahara, A., Transcriptional regulation of a myeloid-lineage specific gene lysozyme C during zebrafish myelopoiesis, 314-323, Copyright (2009) with permission from Elsevier. Full text @ Mech. Dev.