FIGURE

Fig. 4

ID

ZDB-FIG-090304-47

Publication

Martin et al., 2009 - Plakoglobin has both structural and signalling roles in zebrafish development

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Fig. 4

Expression of cardiac marker genes in plakoglobin morphant and control morpholino injected embryos. (A and B) lateral views of 24 hpf and (C–J) ventral views of 48 hpf wholemount embryos. (A) Nkx2.5 at 24 hpf was expressed to the left of the midline in control injected embryos. (B) Nkx2.5 expression is reduced in morphant embryos but in the correct location. (C) Cmlc2 is expressed in both atrium and ventricle in 48 hpf control embryos and there is decreased expression in (D) morphant embryos. (E) Vmhc delineates ventricle specification at 48 hpf and (F) is decreased in morphant embryos. (G and I) Expression of both notch1b and bmp4 expression was restricted to the atrio-ventricular boundary (black arrows) in control morpholino injected embryo hearts. (H and J) In morphant embryos, both cardiac valve markers were expressed throughout the heart and were not restricted to the atrio-ventricular boundary (white arrows). The numbers of embryos with the displayed phenotype were (A) 62/65 (B) 55/63 (C) 72/75 (D) 53/72 (E) 68/70 (F) 40/72,(G) 77/80, (H) 67/85, (I) 74/78 and (J) 68/74.

Expression Data

Genes:	bmp4 myh7 myl7 nkx2.5 notch1b
Fish:	AB AB + MO1-jupa
Knockdown Reagent:	MO1-jupa
Anatomical Terms:	atrium cardiac ventricle heart primitive heart tube
Stage Range:	Prim-5 to Long-pec

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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Reprinted from Developmental Biology, 327(1), Martin, E.D., Moriarty, M.A., Byrnes, L., and Grealy, M., Plakoglobin has both structural and signalling roles in zebrafish development, 83-96, Copyright (2009) with permission from Elsevier. Full text @ Dev. Biol.