Fig. S1

Loss of plakoglobin increases β-catenin protein expression but does not affect β-catenin RNA expression. (A) Western blot analysis of 72 hpf control- or plakoglobin- morpholino injected embryos using a polyclonal anti-β-catenin antibody showing an increase in β-catenin expression in morphant embryos. An anti α-tubulin antibody was used as a loading control. (B) Graph of relative densitometric units of β-catenin expression normalised to α-tubulin levels in control and morphant embryos. (C–F) β-catenin-1 and β-catenin-2 localisation was examined in sphere stage embryos. Expression of β-catenins was similar in morphant embryos and control injected embryo. (G) Semi-quantitative RT-PCR amplification from 24 hpf plakoglobin and control morpholino injected embryos. Lane order: 100 bp ladder, PCR cycle 13, 18, 23, 28, 33, positive plasmid control and negative template control. β-actin was used as a template control. β-catenin-1 or -2 RNA expression in morphant embryos were similar to control-morpholino injected embryos.