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ZFIN ID: ZDB-FIG-080918-35
Alexander et al., 1999 - casanova plays an early and essential role in endoderm formation in zebrafish. Developmental Biology   215(2):343-357 Full text @ Dev. Biol.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Anatomical Terms:
Stage: 20-25 somites
PHENOTYPE:
Fish:
Observed In:
Stage: 20-25 somites

Fig. 2 cas mutants do not express molecular markers of endoderm differentiation. Wild-type (A, C, E) and cas mutant (B, D, F) embryos were examined at the 25-somite stage (21.5 hpf) for expression of axial (A, B), fkd7 (C, D), and gata4 (E, F). (A) axial is expressed in the ventral neuroectoderm and anterior endoderm (arrowhead); endodermal axial expression is absent from cas mutants (B). Similarly, fkd7 and gata4 are expressed in the endoderm of wild-type (arrowheads in C, E) but not cas mutant (D, F) embryos. fkd7 is also expressed in the floor plate and hypochord (C), which is posteriorly shortened in cas mutants (arrow in D). Endodermal gata4 expression defines a region of the gut tube that will form the liver. Myocardial gata4 expression (arrow in E) illustrates the cardia bifida present in cas mutants (arrows in F). (A–D) Lateral views, anterior to the left, dorsal to the top; (E, F) dorsal views, anterior to the left.

Gene Expression Details
Gene Antibody Fish Conditions Stage Qualifier Anatomy Assay
foxa1 WT standard conditions 20-25 somites endoderm ISH
20-25 somites floor plate ISH
20-25 somites hypochord ISH
sox32ta56/ta56 standard conditions 20-25 somites Not Detected endoderm ISH
20-25 somites floor plate ISH
20-25 somites hypochord ISH
foxa2 WT standard conditions 20-25 somites endoderm anterior region ISH
20-25 somites neuroectoderm ventral region ISH
sox32ta56/ta56 standard conditions 20-25 somites Not Detected endoderm ISH
20-25 somites neuroectoderm ventral region ISH
foxa3 WT standard conditions 20-25 somites endoderm ISH
sox32ta56/ta56 standard conditions 20-25 somites Not Detected endoderm ISH
gata4 WT standard conditions 20-25 somites cardiac muscle myoblast ISH
20-25 somites endoderm ISH
sox32ta56/ta56 standard conditions 20-25 somites cardiac muscle myoblast ISH
20-25 somites Not Detected endoderm ISH
20-25 somites Not Detected gut ISH
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
sox32ta56/ta56 standard conditions 20-25 somites cardioblast migration to the midline involved in heart field formation disrupted, abnormal
20-25 somites endoderm absent, abnormal
20-25 somites endoderm formation disrupted, abnormal
20-25 somites heart rudiment split, abnormal
20-25 somites hypochord decreased length, abnormal
Acknowledgments:
ZFIN wishes to thank the journal Developmental Biology for permission to reproduce figures from this article. Please note that this material may be protected by copyright.

Reprinted from Developmental Biology, 215(2), Alexander, J., Rothenberg, M., Henry, G.L., and Stainier, D.Y.R., casanova plays an early and essential role in endoderm formation in zebrafish, 343-357, Copyright (1999) with permission from Elsevier. Full text @ Dev. Biol.