PUBLICATION

casanova plays an early and essential role in endoderm formation in zebrafish

Authors

Alexander, J., Rothenberg, M., Henry, G.L., and Stainier, D.Y.R.

ID

ZDB-PUB-991102-11

Date

1999

Source

Developmental Biology 215(2): 343-357 (Journal)

Registered Authors

Alexander, Jon, Stainier, Didier

Keywords

gut; axial; gata5; mixer; forerunner cells; Kupffer's vesicle; cardia bifida; endothelium; knypek; one-eyed pinhead

MeSH Terms

Amino Acid Sequence
Zebrafish/embryology*
Zebrafish/genetics*
Zebrafish Proteins*
Transcription Factors/genetics
Morphogenesis/genetics
Forkhead Transcription Factors
Genes, Homeobox
Animals
Endoderm/physiology*
Mesoderm/physiology
Gastrula/physiology
Cell Differentiation
Molecular Sequence Data

(all 14)

PubMed

10545242 Full text @ Dev. Biol.

Abstract

The cellular and molecular mechanisms that regulate endoderm development in vertebrates have only recently begun to be explored. Here we show that the zebrafish locus casanova plays an early and essential role in this process. casanova mutants lack a gut tube and do not express any molecular markers of endoderm differentiation. The early endodermal expression of genes such as axial, gata5, and fkd2 does not initiate in casanova mutants, indicating that the endoderm is defective from the onset of gastrulation. Mosaic analysis demonstrates that casanova functions cell autonomously within the endodermal progenitors. We also report the isolation of a zebrafish homologue of Mixer, a gene important for early endoderm formation in Xenopus. casanova does not encode zebrafish Mixer, and mixer expression is normal in casanova mutants, indicating that casanova acts downstream of, or parallel to, mixer to promote endoderm formation. We further find that the forerunner cells, a specialized group of noninvoluting dorsal mesendodermal cells, do not form in casanova mutants. Studies of casanova mutants do not support an important role for the forerunner cells in either dorsal axis or tail development, as has been previously proposed. In addition, although different populations of mesodermal precursors are generated normally in casanova mutants, morphogenetic defects in the heart, vasculature, blood, and kidney are apparent, suggesting a possible role for the endoderm in morphogenesis of these organs.

Genes / Markers

Figures

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Expression

Gene	Fish	Conditions	Stage	Qualifier	Anatomy	Assay	Figure
foxa1	sox32^ta56/ta56	standard conditions	20-25 somites	Not Detected	endoderm	ISH	Fig. 2
foxa1	sox32^ta56/ta56	standard conditions	20-25 somites		floor plate	ISH	Fig. 2
foxa1	sox32^ta56/ta56	standard conditions	20-25 somites		hypochord	ISH	Fig. 2
foxa1	WT	standard conditions	20-25 somites		endoderm	ISH	Fig. 2
foxa1	WT	standard conditions	20-25 somites		floor plate	ISH	Fig. 2
foxa1	WT	standard conditions	20-25 somites		hypochord	ISH	Fig. 2
foxa2	gpc4^m119/m119	standard conditions	Prim-5		endoderm	ISH	Fig. 7
foxa2	sox32^ta56/ta56	standard conditions	75%-epiboly		axial chorda mesoderm	ISH	Fig. 3
foxa2	sox32^ta56/ta56	standard conditions	20-25 somites	Not Detected	endoderm	ISH	Fig. 2
foxa2	sox32^ta56/ta56	standard conditions	75%-epiboly	Not Detected	endoderm	ISH	Fig. 3

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Phenotype

Fish	Conditions	Stage	Phenotype	Figure
gpc4^m119/m119	standard conditions	Prim-5	convergent extension involved in gastrulation disrupted, abnormal	Fig. 7
gpc4^m119/m119	standard conditions	Prim-5	endoderm mislocalised, abnormal	Fig. 7
gpc4^m119/m119	standard conditions	Prim-5	endothelial cell mislocalised, abnormal	Fig. 7
sox32^ta56/ta56	standard conditions	Shield	endoderm absent, abnormal	Fig. 3
sox32^ta56/ta56	standard conditions	Shield	endoderm formation disrupted, abnormal	Fig. 3
sox32^ta56/ta56	standard conditions	Shield	forerunner cell group absent, abnormal	Fig. 6
sox32^ta56/ta56	standard conditions	Shield	Kupffer's vesicle absent, abnormal	Fig. 6
sox32^ta56/ta56	standard conditions	90%-epiboly	forerunner cell group absent, abnormal	Fig. 6
sox32^ta56/ta56	standard conditions	90%-epiboly	Kupffer's vesicle absent, abnormal	Fig. 6
sox32^ta56/ta56	standard conditions	5-9 somites	Kupffer's vesicle absent, abnormal	Fig. 6

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Mutations / Transgenics

Allele	Construct	Type	Affected Genomic Region
m119		Point Mutation	gpc4
ta56		Point Mutation	sox32

Human Disease / Model

Sequence Targeting Reagents

Fish

Fish
gpc4^m119/m119
sox32^ta56/ta56
WT

Antibodies

Orthology

Engineered Foreign Genes

Mapping

Alexander et al., 1999 ZDB-PUB-991102-11 PMID:10545242

casanova plays an early and essential role in endoderm formation in zebrafish

Alexander et al., 1999

ZDB-PUB-991102-11
PMID:10545242