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ZFIN ID: ZDB-FIG-080918-40
Alexander et al., 1999 - casanova plays an early and essential role in endoderm formation in zebrafish. Developmental Biology   215(2):343-357 Full text @ Dev. Biol.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Gene:
Fish:
Anatomical Terms:
Stage Range: Shield to 75%-epiboly
PHENOTYPE:
Fish:
Observed In:
Stage Range: Shield to 5-9 somites

Fig. 6 The forerunner cells do not form in cas mutants. Wildtype and cas mutant embryos were examined by light microscopy (A, B), in situ hybridization for expression of ntl (C, D), syto-11 fluorescence (E, F), and Nomarski optics (G, H). Embryos are at the following stages: (A, B) 6-somite, (C, D) 70% epiboly, (E, F) 90% epiboly, and (G, H) shield. Kupffer’s vesicle, formed by the forerunner cells (FRs), is easily seen in the tail of wild-type embryos (arrowhead in A) but not in cas mutants (B). ntl is normally expressed in the FRs (arrowhead in C) but this expression is lacking in cas mutants (D). The FR cluster (arrowhead in E) can be visualized by labeling with the fluorescent dye syto-11; cells of the enveloping layer (EVL) also take up syto-11 and thus fluoresce. cas mutants appear to lack a FR cluster (F); several syto-11-labeled EVL cells are seen. Using Nomarski optics the FRs can be directly visualized at the shield stage as a cluster of cells (arrowheads) that obscures part of the dorsal margin (arrows). No FRs are seen in a cas mutant embryo (H), allowing the margin to be easily traced (arrows). (A, B, E, F) Posterior views, dorsal to the right; (C, D, G, H) dorsal views, anterior to the top.

Gene Expression Details
Gene Antibody Fish Conditions Stage Qualifier Anatomy Assay
tbxta WT standard conditions Shield forerunner cell group ISH
Shield germ ring ISH
Shield margin ISH
75%-epiboly forerunner cell group ISH
75%-epiboly germ ring ISH
75%-epiboly margin ISH
75%-epiboly prechordal plate ISH
sox32ta56/ta56 standard conditions Shield Not Detected forerunner cell group ISH
Shield germ ring ISH
Shield margin ISH
75%-epiboly Not Detected forerunner cell group ISH
75%-epiboly germ ring ISH
75%-epiboly margin ISH
75%-epiboly prechordal plate ISH
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
sox32ta56/ta56 standard conditions Shield forerunner cell group absent, abnormal
Shield Kupffer's vesicle absent, abnormal
90%-epiboly forerunner cell group absent, abnormal
90%-epiboly Kupffer's vesicle absent, abnormal
5-9 somites Kupffer's vesicle absent, abnormal
Acknowledgments:
ZFIN wishes to thank the journal Developmental Biology for permission to reproduce figures from this article. Please note that this material may be protected by copyright.

Reprinted from Developmental Biology, 215(2), Alexander, J., Rothenberg, M., Henry, G.L., and Stainier, D.Y.R., casanova plays an early and essential role in endoderm formation in zebrafish, 343-357, Copyright (1999) with permission from Elsevier. Full text @ Dev. Biol.