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ZFIN ID: ZDB-FIG-080918-41
Alexander et al., 1999 - casanova plays an early and essential role in endoderm formation in zebrafish. Developmental Biology   215(2):343-357 Full text @ Dev. Biol.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Anatomical Terms:
Stage Range: 20-25 somites to Prim-5
PHENOTYPE:
Fish:
Observed In:
Stage Range: 20-25 somites to Prim-5

Fig. 7 Morphogenesis of several mesodermal derivatives is defective in cas mutants. Wild-type (A, B, C, D), cas (E, F, G, H), and kny (I, J, K, L) embryos showing expression of nkx2.5 (A, E, I), tie2 (B, F, J), gata1 (C, G), pax2.1 (D, H), and axial (K, L). Embryos are at 24 (A, E, I–L) and 21.5 hpf (B–D, F–H). By 24 hpf the definitive heart tube has formed in wild-type embryos (A) while two “hearts” are evident in cas mutants (E). Pharyngeal endodermal expression of nkx2.5 is also missing in cas mutants (compare A to E). Endothelial precursors assemble smoothly in the midline of wild-type (B) but not cas mutant (F) embryos. In wild-type embryos the bilateral red blood cell precursors meet at the midline (C), but are positioned more laterally in cas mutants (G). The bilateral pronephroi (arrowheads) and pronephric ducts are also positioned more laterally in cas mutants (compare D to H); the arrows indicate the pax2.1-expressing spinal commissural interneurons. kny mutants form endoderm normally, although it is more broadly distributed (K, L); they do not exhibit cardia bifida (I); and their trunk endothelium appears smoothly although again more broadly arranged in the midline (J). All are dorsal views, anterior to the left, except (K), which is a lateral view, anterior to the left.

Gene Expression Details
Gene Antibody Fish Conditions Stage Qualifier Anatomy Assay
foxa2 gpc4m119/m119 standard conditions Prim-5 endoderm ISH
nkx2.5 WT standard conditions Prim-5 heart tube ISH
Prim-5 pharyngeal endoderm ISH
gpc4m119/m119 standard conditions Prim-5 heart tube ISH
Prim-5 Not Detected pharyngeal endoderm ISH
sox32ta56/ta56 standard conditions Prim-5 heart tube ISH
Prim-5 Not Detected pharyngeal endoderm ISH
pax2a WT standard conditions 20-25 somites pronephric duct ISH
20-25 somites pronephros ISH
20-25 somites spinal cord interneuron ISH
sox32ta56/ta56 standard conditions 20-25 somites pronephric duct ISH
20-25 somites pronephros ISH
20-25 somites spinal cord interneuron ISH
tek WT standard conditions 20-25 somites endothelial cell ISH
gpc4m119/m119 standard conditions Prim-5 endothelial cell ISH
sox32ta56/ta56 standard conditions 20-25 somites endothelial cell ISH
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
gpc4m119/m119 standard conditions Prim-5 convergent extension involved in gastrulation disrupted, abnormal
Prim-5 endoderm mislocalised, abnormal
Prim-5 endothelial cell mislocalised, abnormal
sox32ta56/ta56 standard conditions 20-25 somites blood vessel development disrupted, abnormal
20-25 somites cardioblast migration to the midline involved in heart field formation disrupted, abnormal
20-25 somites endoderm mislocalised, abnormal
20-25 somites erythroblast mislocalised laterally, abnormal
20-25 somites kidney morphogenesis disrupted, abnormal
20-25 somites pronephric duct absent, abnormal
20-25 somites pronephric duct mislocalised laterally, abnormal
Acknowledgments:
ZFIN wishes to thank the journal Developmental Biology for permission to reproduce figures from this article. Please note that this material may be protected by copyright.

Reprinted from Developmental Biology, 215(2), Alexander, J., Rothenberg, M., Henry, G.L., and Stainier, D.Y.R., casanova plays an early and essential role in endoderm formation in zebrafish, 343-357, Copyright (1999) with permission from Elsevier. Full text @ Dev. Biol.